Electron and light microscopy of pancreatic islets of Langerhans isolated by Trowell's T8 medium.

The isolation and purification of high yields of islets of Langerhans are important areas of investigation. Although it is well established that warm and cold ischemia affect islet yield and function, little information is available about the damage that occurs during the digestion period. We used Trowell's T8 medium and conventional Hank's balanced salt solution to obtain high yields of islets with morphologically-intact cells. Light and electron microscopy were used to characterize the islets isolated by the two media. Results reveal that the islets isolated by Trowells T8 medium were less fragmented and had better fine-structural integrity than those isolated by the conventional medium.