Tachibana Y
Department of Orthopaedic Surgery, Saitama Medical School, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1990 Jul;64(7):583-92.
In twenty rabbits with completion of bone growth, the medial meniscus was replaced with an allograft meniscus that had been preserved at -80 degrees C for 6 to 14 days. As a control, the medial meniscus was removed and reattached without cryopreservation in 14 rabbits. Replaced menisci were examined histologically and microangiographically. Allografted menisci were found to attach to the capsule at 4 weeks. However, the attachments of the anterior and posterior horns were not stable in the majority of rabbits, because the nylon thread for anchoring was broken in these cases. There was osteophyte formation on the medial tibial plateau and the medial femoral condyle. Histological examinations revealed proliferation of vascularized synovial tissue along the meniscal surface and invasion of para-meniscal connective tissue into the meniscal substance. Chondrocytes showed regeneration in the superficial layer at 12 weeks. This study has demonstrated that cryopreserved meniscal allograft was capable of regeneration. The osteophyte formation of the medial femoral condyle and the tibial plateau appears to be produced by the abnormally unstable transplanted meniscus resulting from the insufficient anchorage of anterior and/or posterior horns.
在20只骨骼生长已完成的兔子中，将内侧半月板替换为在-80℃保存6至14天的同种异体半月板。作为对照，在14只兔子中切除内侧半月板并在未进行冷冻保存的情况下重新附着。对替换的半月板进行组织学和微血管造影检查。发现同种异体半月板在4周时附着于关节囊。然而，在大多数兔子中，前后角的附着并不稳定，因为在这些情况下用于固定的尼龙线断裂。在内侧胫骨平台和内侧股骨髁有骨赘形成。组织学检查显示血管化滑膜组织沿半月板表面增殖，半月板旁结缔组织侵入半月板实质。在12周时软骨细胞在表层显示再生。本研究表明，冷冻保存的同种异体半月板能够再生。内侧股骨髁和胫骨平台的骨赘形成似乎是由前后角固定不足导致的移植半月板异常不稳定所产生的。
