Ako Research Institute, Otsuka Pharmaceutical Co, Ltd, Ako, Hyogo, Japan.
J Ocul Pharmacol Ther. 2012 Jun;28(3):264-70. doi: 10.1089/jop.2011.0147. Epub 2012 Feb 3.
The effects of rebamipide on the number of periodic acid Schiff reagent (PAS)-positive cells in the conjunctiva, the mucin content in the cornea and conjunctiva of normal rabbits, and desiccation-induced corneal damage in vivo were examined.
Rebamipide (0.1%-3%) was applied 6 times a day for 14 days, and the PAS-positive cell count in the bulbar conjunctiva was measured by impression cytology. The amount of conjunctival and corneal mucin-like substances was measured by Alcian blue binding. The corneal damage model was created by desiccation from air flow at room temperature. The level of corneal damage was determined by scoring the area stained with rose bengal and fluorescein dye.
Rebamipide increased the number of PAS-positive cells in the conjunctiva when instilled at concentrations of 0.3% or higher, and 1% rebamipide increased the amount of mucin-like substances of the conjunctiva and cornea. Moreover, 1% rebamipide was also found to lower the rose bengal scores of the cornea in the corneal damage model by desiccation.
Rebamipide is a possible candidate drug for treatment of cornea and conjunctival epithelial damage due to its mucin-like substance increasing action, for instance, in the treatment of dry eye disease.
观察瑞巴派特对正常兔结膜周期性酸希夫试剂（PAS）阳性细胞数、角膜和结膜粘蛋白含量以及体内干燥诱导性角膜损伤的影响。
瑞巴派特（0.1%-3%）每天滴注 6 次，共 14 天，采用印迹细胞学测量球结膜 PAS 阳性细胞计数。采用阿利新蓝结合法测量结膜和角膜粘蛋白样物质的含量。通过室温下空气流干燥来创建角膜损伤模型。通过对孟加拉玫瑰红和荧光素染料染色区域进行评分来确定角膜损伤程度。
瑞巴派特以 0.3%或更高浓度滴眼时，可增加结膜 PAS 阳性细胞数，1%瑞巴派特可增加结膜和角膜粘蛋白样物质的含量。此外，在干燥诱导的角膜损伤模型中，1%瑞巴派特还可降低角膜的孟加拉玫瑰红评分。
瑞巴派特可能是一种治疗角膜和结膜上皮损伤的候选药物，例如，在治疗干眼症方面。
