Guo Chun-yan, Li Hui-jin, Liu Yang, Zhao Xiang-rong, Wang Xin, Feng Qing, Li Yuan, Hu Jun
Xi'an Jiaotong University College of Medicine, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Feb;28(2):177-80.
Preparation of H1 subtype of influenza virus HA protein monoclonal antibody (mAbs), and to analyse their reactivity.
H1N1 influenza virus vaccine (2009) and seasonal A1 influenza virus vaccine as antigen, conventional immunization, fusion, cloning, access to the antigen-specific mAbs. To study the reactivity and specificity of mAbs by ELISA, HI test and Western blot.
We have obtained 97 hybridoma of stable secreting anti-H1 subtype of influenza virus HA protein. According to their different reactivity, these mAbs can be divided into four categories: 39 strains for strain-specific, of which 29 have HI activity; 7 strains for subtype-specific, of which 5 have HI activity; 16 strains for the 2009 pandemic strain and the seasonal strains to common antigens, of which 9 have HI activity; 35 strains common for influenza virus antigens, of which 22 have HI activity.
Both vaccines have better immunogenicity and immune protection activity. Preparation of four types antibody against HA protein of H1 subtype of influenza virus, can provide experimental data for preparation subtype-specific diagnostic kits and influenza A and B virus diagnostic kit, and lay a foundation for further studying of H1N1 influenza virus HA epitope.
制备甲型流感病毒H1亚型血凝素（HA）蛋白单克隆抗体（mAbs），并分析其反应活性。
以2009年甲型H1N1流感病毒疫苗和季节性甲型流感病毒疫苗作为抗原，采用常规免疫、细胞融合、克隆技术，获得针对该抗原的特异性mAbs。通过酶联免疫吸附测定（ELISA）、血凝抑制试验（HI）及蛋白质免疫印迹法（Western blot）研究mAbs的反应活性及特异性。
获得97株稳定分泌抗甲型流感病毒H1亚型HA蛋白的杂交瘤细胞株。根据其反应活性不同，这些mAbs可分为四类：39株为株特异性，其中29株具有HI活性；7株为亚型特异性，其中5株具有HI活性；16株对2009年大流行株和季节性毒株的共同抗原有反应，其中9株具有HI活性；35株对流感病毒抗原有共同反应，其中22株具有HI活性。
两种疫苗均具有较好的免疫原性和免疫保护活性。制备的针对甲型流感病毒H1亚型HA蛋白的四类抗体，可为制备亚型特异性诊断试剂盒及甲型和乙型流感病毒诊断试剂盒提供实验数据，为进一步研究甲型H1N1流感病毒HA表位奠定基础。
