Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Comp Immunol Microbiol Infect Dis. 2012 Mar;35(2):209-16. doi: 10.1016/j.cimid.2012.01.007. Epub 2012 Feb 2.
Mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, is highly prevalent worldwide and causes major economic losses to the pig industry. Commercial vaccines are widely used in the control of this disease, however, they provide only partial protection. The aim of this study was to evaluate 34 recombinant proteins of M. hyopneumoniae expressed in Escherichia coli. Antigenic and immunogenic properties of these proteins were analyzed. For this, the proteins were tested against hyperimmune and convalescent pig sera through ELISA and Western blot. Immunogenicity of the recombinant proteins was evaluated in BALB/c mice following intramuscular inoculation. Most antigens were able to induce a strong immune response and sera from inoculated mice were able to recognize native proteins by cell ELISA and Western blot. Several recombinant proteins were specifically recognized by convalescent pig sera, indicating they are expressed during infection. These data may help to develop more efficacious vaccines against M. hyopneumoniae.
猪肺炎支原体是地方性肺炎的主要病原体，在世界范围内广泛流行，给养猪业造成重大经济损失。商业疫苗被广泛用于该病的防控，但只能提供部分保护。本研究旨在评估 34 种在大肠杆菌中表达的猪肺炎支原体重组蛋白，分析这些蛋白的抗原性和免疫原性。为此，通过 ELISA 和 Western blot 检测了这些蛋白与高免血清和康复血清的反应性。通过肌肉接种评估了重组蛋白在 BALB/c 小鼠中的免疫原性。大多数抗原能够诱导强烈的免疫反应，接种小鼠的血清能够通过细胞 ELISA 和 Western blot 识别天然蛋白。一些重组蛋白被康复血清特异性识别，表明它们在感染过程中表达。这些数据可能有助于开发针对猪肺炎支原体的更有效的疫苗。
