Khan Junaid A, Abdul Rahman M N A, Mazari F A K, Shahin Y, Smith G, Madden L, Fagan M J, Greenman J, McCollum P T, Chetter I C
Academic Vascular Surgical Unit, Hull York Medical School and University of Hull, Hull, UK.
Ann Vasc Surg. 2012 Apr;26(3):322-9. doi: 10.1016/j.avsg.2011.08.015. Epub 2012 Feb 4.
The influence of intraluminal thrombus (ILT) on the proteolytic environment within the wall of an abdominal aortic aneurysm (AAA) is unknown. This is the first study to examine the correlation between ILT thickness and the levels of matrix metalloproteinases (MMPs) and their natural inhibitors (tissue inhibitors of matrix metalloproteinases [TIMPs]) within the adjacent AAA wall.
Thirty-five patients undergoing elective repair of AAAs were studied. A single full-thickness infrarenal aortic sample was obtained uniformly from the arteriotomy site from each patient. All samples were snap frozen and analyzed for total and active MMP 2, 8, and 9 and TIMP 1 and 2. Thrombus thickness at the specimen site was measured on the preoperative contrast computed tomographic angiograms.
There was a statistically significant correlation between ILT thickness, concentration of TIMP 1, and active concentration of MMP 9. MMP 2 (active and total) and TIMP 2 demonstrated a positive correlation with ILT thickness, although not statistically significant.
In this novel study, we found a significant positive correlation of ILT thickness with active MMP 9 and TIMP 1 concentration in the adjacent AAA wall, and this may have implications for AAA expansion and eventual rupture.
腔内血栓（ILT）对腹主动脉瘤（AAA）壁内蛋白水解环境的影响尚不清楚。这是第一项研究ILT厚度与相邻AAA壁内基质金属蛋白酶（MMPs）及其天然抑制剂（基质金属蛋白酶组织抑制剂[TIMPs]）水平之间相关性的研究。
对35例行AAA择期修复术的患者进行研究。从每位患者的动脉切开部位均匀获取单个全层肾下腹主动脉样本。所有样本均速冻，并分析MMP 2、8和9以及TIMP 1和2的总量和活性。在术前对比增强计算机断层血管造影上测量标本部位的血栓厚度。
ILT厚度、TIMP 1浓度和MMP 9活性浓度之间存在统计学显著相关性。MMP 2（活性和总量）和TIMP 2与ILT厚度呈正相关，尽管无统计学意义。
在这项新研究中，我们发现ILT厚度与相邻AAA壁内活性MMP 9和TIMP 1浓度呈显著正相关，这可能对AAA扩张和最终破裂有影响。
