On-line sample pre-concentration in microfluidic devices: a review.

On-line sample preconcentration is an essential tool in the development of microfluidic-based separation platforms. In order to become more competitive with traditional separation techniques, the community must continue to develop newer and more novel methods to improve detection limits, remove unwanted sample matrix components that disrupt separation performance, and enrich/purify analytes for other chip-based actions. Our goal in this review is to familiarize the reader with many of the options available for on-chip concentration enhancement with a focus on those manuscripts that, in our assessment, best describe the fundamental principles that govern those enhancements. Sections discussing both electrophoretic and nonelectrophoretic modes of preconcentration are included with a focus on device design and mechanisms of preconcentration. This review is not meant to be a comprehensive collection of every available example, but our hope is that by learning how on-line sample concentration techniques are being applied today, the reader will be inspired to apply these techniques to further enhance their own programs.