The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
Fitoterapia. 2012 Jul;83(5):831-7. doi: 10.1016/j.fitote.2012.01.007. Epub 2012 Jan 27.
Polyprenols with various pharmacological activities were first isolated from Taxus chinensis var. mairei, which is native to China, and were identified by a high performance liquid chromatography/mass spectrometry (HPLC/MS), (1)H nuclear magnetic resonance ((1)H NMR), (13)C NMR and infrared (IR) spectroscopy. The taxus polyprenols (TPs) had two maxima of polyprenol-17 and polyprenol-21 and different from polyprenols distributed in other plant species. A reversed-phase HPLC method with a simple gradient elution was established, and the proposed combined peak area method for the quantification of polyprenols was verified. The analysis indicated that TPs were present in the old needles at levels as high as 3% and that T. chinensis var. mairei could be an alternative botanical source for the extraction of polyprenols.
从原产于中国的中国红豆杉中首次分离出具有多种药理活性的多萜醇，并用高效液相色谱/质谱（HPLC/MS）、（1）H 核磁共振（（1）H NMR）、（13）C 核磁共振和红外（IR）光谱对其进行了鉴定。红豆杉多萜醇（TPs）在多萜醇 17 和多萜醇 21 处有两个最大值，与分布在其他植物物种中的多萜醇不同。建立了一种简单梯度洗脱的反相高效液相色谱法，并验证了所提出的用于定量多萜醇的组合峰面积法。分析表明，TPs 在老针叶中的含量高达 3%，因此中国红豆杉可以作为提取多萜醇的替代植物来源。
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