采用高效液相色谱法比较曼地亚红豆杉和南方红豆杉不同部位的紫杉醇含量。
Taxol content comparison in different parts of Taxus madia and Taxus chinensis var. mairei by HPLC.
作者信息
Dong Quan-Feng, Liu Jing-Jing, Yu Rong-Min
机构信息
College of Pharmacy, Jinan University, Guangzhou 510632, China.
出版信息
Zhong Yao Cai. 2010 Jul;33(7):1048-51.
OBJECTIVE
To analysis and compare the taxol content in different parts of Taxus madia and Taxus chinensis var. mairei by High Performance Liquid Chromatography (HPLC).
METHODS
85% EtOH and CH2Cl2 were used for the extraction of taxol. By HPLC, the methodology study and taxol content investigation were performed.
RESULTS
The taxol was extracted successfully. One simple and reliable methodology was built up. Basing on these, the taxol content in these two Taxus spp. were analysed and compared, among of which the leaf of Taxus chinensis var. mairei has the highest taxol content (5.18 x 10(-5), w/w).
CONCLUSION
Taxol and its content in the original plants can be simply and reliably extracted and investigated by these methods, which also can provided the scientific basis for the rational development of Taxus spp.
目的
采用高效液相色谱法（HPLC）分析和比较曼地亚红豆杉和南方红豆杉不同部位的紫杉醇含量。
方法
用85%乙醇和二氯甲烷提取紫杉醇。通过HPLC进行方法学研究和紫杉醇含量测定。
结果
成功提取出紫杉醇。建立了一种简单可靠的方法。在此基础上，对这两种红豆杉属植物中的紫杉醇含量进行了分析和比较，其中南方红豆杉叶片的紫杉醇含量最高（5.18×10⁻⁵，w/w）。
结论
通过这些方法可以简单可靠地提取和测定原植物中的紫杉醇及其含量，也可为红豆杉属植物的合理开发提供科学依据。
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