van Ginkel M F, van der Voet G B, van Eijk H G, de Wolff F A
Laboratorium voor Toxicologie, Academisch Ziekenhuis Leiden, Nederland.
J Clin Chem Clin Biochem. 1990 Jul;28(7):459-63. doi: 10.1515/cclm.1990.28.7.459.
The binding of aluminium in rat serum was studied. Rats were loaded intraperitoneally with different doses of aluminium(III)chloride 4 times during one week, before being killed by cardiac puncture. One ml of serum was applied to a Sephacryl S-200 SF column and 70 fractions were collected. In the collected fractions, the distribution of aluminium was measured and compared with the concentrations of total protein, transferrin, and citrate. The presence of a high molecular weight aluminium-complex in serum is confirmed. Although a possible role for albumin cannot be excluded, it is most likely that transferrin plays a role as a carrier for biological transport of aluminium in plasma. In addition to transferrin, aluminium was shown to be associated with citrate in serum, resulting in a low-molecular weight complex. It is postulated that citrate acts as a chelator for aluminium, and that the Al/citrate complex in serum may play an important role in intracellular accumulation, and hence the toxicity, of aluminium.
研究了大鼠血清中铝的结合情况。在一周内给大鼠腹腔注射不同剂量的氯化铝（III）4次，然后通过心脏穿刺处死。将1毫升血清应用于Sephacryl S - 200 SF柱，并收集70个馏分。在收集的馏分中，测量铝的分布，并与总蛋白、转铁蛋白和柠檬酸盐的浓度进行比较。证实血清中存在高分子量铝复合物。虽然不能排除白蛋白可能发挥的作用，但转铁蛋白很可能作为铝在血浆中生物转运的载体发挥作用。除转铁蛋白外，还发现铝与血清中的柠檬酸盐相关，形成低分子量复合物。据推测，柠檬酸盐作为铝的螯合剂，血清中的铝/柠檬酸盐复合物可能在铝的细胞内积累及其毒性方面发挥重要作用。
