Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin.
作者信息
Farace C, Blanchot B, Champiat D, Couble P, Declercq G, Millet J L
机构信息
CEMAGREF, Laboratoire de Biochimie, Lyon, France.
出版信息
J Clin Chem Clin Biochem. 1990 Jul;28(7):471-4. doi: 10.1515/cclm.1990.28.7.471.
PMID:2230665
Abstract
A derivative of luciferin, 4-methyl-D-luciferin, was synthesized in low yield, and characterized by its ultraviolet and fluorescence spectra. The spectra of luciferin and its derivative are very similar. 4-Methyl-D-luciferin is a substrate of Photinus pyralis luciferase, and it causes light emission in luminometric assays.
摘要
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