• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin.

作者信息

Farace C, Blanchot B, Champiat D, Couble P, Declercq G, Millet J L

机构信息

CEMAGREF, Laboratoire de Biochimie, Lyon, France.

出版信息

J Clin Chem Clin Biochem. 1990 Jul;28(7):471-4. doi: 10.1515/cclm.1990.28.7.471.

DOI:10.1515/cclm.1990.28.7.471
PMID:2230665
Abstract

A derivative of luciferin, 4-methyl-D-luciferin, was synthesized in low yield, and characterized by its ultraviolet and fluorescence spectra. The spectra of luciferin and its derivative are very similar. 4-Methyl-D-luciferin is a substrate of Photinus pyralis luciferase, and it causes light emission in luminometric assays.

摘要

相似文献

1
Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin.
J Clin Chem Clin Biochem. 1990 Jul;28(7):471-4. doi: 10.1515/cclm.1990.28.7.471.
2
Fluorescent properties of firefly luciferases and their complexes with luciferin.萤火虫荧光素酶及其与荧光素复合物的荧光特性。
Biosci Rep. 2000 Feb;20(1):21-30. doi: 10.1023/a:1005579016387.
3
Development of near-infrared firefly luciferin analogue reacted with wild-type and mutant luciferases.近红外萤光素类似物与野生型和突变型萤光酶的反应开发。
Chirality. 2020 Jul;32(7):922-931. doi: 10.1002/chir.23236. Epub 2020 May 4.
4
A novel bioluminogenic assay for alpha-chymotrypsin.一种用于α-糜蛋白酶的新型生物发光测定法。
J Biolumin Chemilumin. 1995 Jul-Aug;10(4):213-8. doi: 10.1002/bio.1170100404.
5
Bioluminescence of beetle luciferases with 6'-amino-D-luciferin analogues reveals excited keto-oxyluciferin as the emitter and phenolate/luciferin binding site interactions modulate bioluminescence colors.甲虫荧光素酶与6'-氨基-D-荧光素类似物的生物发光揭示了激发态的酮基氧化荧光素作为发光体，且酚盐/荧光素结合位点相互作用调节生物发光颜色。
Biochemistry. 2014 Aug 19;53(32):5208-20. doi: 10.1021/bi500160m. Epub 2014 Aug 4.
6
Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells.用于在活的完整细胞中检测萤火虫荧光素酶的膜通透性荧光素酯。
Biochem J. 1991 Jun 15;276 ( Pt 3)(Pt 3):637-41. doi: 10.1042/bj2760637.
7
An easily synthesized, photolyzable luciferase substrate for in vivo luciferase activity measurement.一种易于合成的、可光解的荧光素酶底物，用于体内荧光素酶活性测量。
Biotechniques. 1993 Nov;15(5):848-50.
8
The origin of luciferase activity in Zophobas mealworm AMP/CoA-ligase (protoluciferase): luciferin stereoselectivity as a switch for the oxygenase activity.荧光素酶在黄粉虫 AMP/CoA 连接酶（原荧光素酶）中的活性起源：荧光素立体选择性作为氧合酶活性的开关。
Photochem Photobiol Sci. 2010 Aug;9(8):1111-9. doi: 10.1039/c0pp00080a. Epub 2010 Jun 5.
9
Structural evolution of luciferase activity in Zophobas mealworm AMP/CoA-ligase (protoluciferase) through site-directed mutagenesis of the luciferin binding site.通过突变荧光素结合位点对黄粉虫 AMP/CoA-连接酶（原荧光素酶）的荧光酶活性进行结构演化。
Photochem Photobiol Sci. 2011 Jul;10(7):1226-32. doi: 10.1039/c0pp00392a. Epub 2011 Apr 19.
10
Luciferase Activity of Insect Fatty Acyl-CoA Synthetases with Synthetic Luciferins.昆虫脂肪酰辅酶A合成酶与合成荧光素的荧光素酶活性
ACS Chem Biol. 2017 Dec 15;12(12):2946-2951. doi: 10.1021/acschembio.7b00813. Epub 2017 Nov 7.

引用本文的文献

1
Lessons Learned from Luminous Luciferins and Latent Luciferases.从发光萤光素和潜伏萤光酶中吸取的教训。
ACS Chem Biol. 2018 Jul 20;13(7):1734-1740. doi: 10.1021/acschembio.7b00964. Epub 2018 Feb 19.
2
Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions.萤火虫荧光素酶：具有多种催化功能的腺苷酸形成酶。
Cell Mol Life Sci. 2010 Feb;67(3):387-404. doi: 10.1007/s00018-009-0170-8. Epub 2009 Oct 27.