Department of Bioengineering, University of California at San Diego, La Jolla, California, USA.
Nat Methods. 2012 Feb 5;9(3):270-2. doi: 10.1038/nmeth.1871.
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
靶向 DNA 甲基化定量分析可快速、经济有效地检测多个样本中最具信息量的基因座。在这里，我们报告了改进的亚硫酸氢盐连接探针（BSPP），其设计算法可生成高效的连接探针，还报告了一种无文库方案，该方案大大降低了样品制备成本和时间，并且与自动化兼容，以及一种高效的生物信息学分析流程，可通过测序准确地获得经亚硫酸氢盐转化的 DNA 的甲基化水平和基因型。
Zotero 插件