Witteveen C F, van de Vondervoort P, Dijkema C, Swart K, Visser J
Department of Genetics, Agricultural University, Wageningen, The Netherlands.
J Gen Microbiol. 1990 Jul;136(7):1299-305. doi: 10.1099/00221287-136-7-1299.
A glycerol-kinase-deficient mutant of Aspergillus niger was isolated. Genetic analysis revealed that the mutation is located on linkage group VI. The phenotype of this mutant differed from that of a glycerol kinase mutant of Aspergillus nidulans in its ability to utilize dihydroxyacetone (DHA). The weak growth on glycerol of the A. niger glycerol kinase mutant showed that glycerol phosphorylation is an important step in glycerol catabolism. The mutant could still grow normally on DHA because of the presence of a DHA kinase. This enzyme, probably in combination with an NAD(+)-dependent glycerol dehydrogenase, present only in the mutant, is responsible for the weak growth of the mutant on glycerol. Enzymic analysis of both the mutant and the parental strain showed that at least three different glycerol dehydrogenases were formed under different physiological conditions: the NAD(+)-dependent enzyme described above, a constitutive NADP(+)-dependent enzyme and a D-glyceraldehyde-specific enzyme induced on D-galacturonate. The glycerol kinase mutant showed impaired growth on D-galacturonate.
分离得到了黑曲霉的甘油激酶缺陷型突变体。遗传分析表明该突变位于第六连锁群上。该突变体的表型与其在利用二羟基丙酮（DHA）能力方面与构巢曲霉的甘油激酶突变体不同。黑曲霉甘油激酶突变体在甘油上生长微弱，这表明甘油磷酸化是甘油分解代谢中的重要步骤。由于存在DHA激酶，该突变体在DHA上仍能正常生长。这种酶可能与仅在突变体中存在的NAD(+)依赖性甘油脱氢酶共同作用，导致突变体在甘油上生长微弱。对突变体和亲本菌株的酶分析表明，在不同生理条件下至少形成了三种不同的甘油脱氢酶：上述的NAD(+)依赖性酶、组成型NADP(+)依赖性酶以及在D-半乳糖醛酸上诱导产生的D-甘油醛特异性酶。甘油激酶突变体在D-半乳糖醛酸上生长受损。
