两种浓核病毒基因组的限制酶切图谱和序列同源性

Restriction maps and sequence homologies of two densovirus genomes.

作者信息

Jousset F X, Jourdan M, Compagnon B, Mialhe E, Veyrunes J C, Bergoin M

机构信息

Station de Recherches de Pathologie, Comparée, INRA-URA 1184 du CNRS, Saint-Christol-lez-Alès, France.

出版信息

J Gen Virol. 1990 Oct;71 ( Pt 10):2463-6. doi: 10.1099/0022-1317-71-10-2463.

DOI:10.1099/0022-1317-71-10-2463

PMID:2230740

Abstract

The genomes of Junonia coenia densonucleosis virus (JcDNV) and Galleria mellonella densonucleosis virus (GmDNV) were analysed by restriction endonuclease analysis and Southern blot hybridization. A total of 37 and 33 restriction sites were mapped on JcDNV and GmDNV DNA, respectively. BglI, HaeII and BstEII were site-specific for JcDNV DNA, and BglII and ClaI for GmDNV DNA. The two genomes had nearly identical maps for several restriction endonucleases and Southern blot hybridization using a total genomic JcDNV probe indicated extensive DNA sequence homologies spanning the entire length of the two genomes. Symmetrical cleavage sites, mapping at the extremities of both genomes, confirmed the presence of inverted terminal repeats of at least 420 to 440 bases in length.

摘要

通过限制性内切酶分析和Southern印迹杂交对苎麻珍蝶浓核症病毒（JcDNV）和大蜡螟浓核症病毒（GmDNV）的基因组进行了分析。分别在JcDNV和GmDNV DNA上定位了总共37个和33个限制性位点。BglI、HaeII和BstEII对JcDNV DNA具有位点特异性，而BglII和ClaI对GmDNV DNA具有位点特异性。这两个基因组对于几种限制性内切酶具有几乎相同的图谱，并且使用全基因组JcDNV探针进行的Southern印迹杂交表明，在两个基因组的全长范围内存在广泛的DNA序列同源性。位于两个基因组末端的对称切割位点证实了至少长度为420至440个碱基的反向末端重复序列的存在。