Brust P, Diemer N H
Department of Cell Biology and Regulation, Karl Marx University, Leipzig, G.D.R.
J Neurochem. 1990 Dec;55(6):2098-104. doi: 10.1111/j.1471-4159.1990.tb05801.x.
To determine whether a previously reported effect of vasopressin on blood-brain transfer of leucine extends to other large neutral amino acids, we measured the regional blood-brain transfer of L-phenylalanine with the integral technique. Intravenous co-injection of L-phenylalanine and arginine vasopressin (30 nmol to 10 pmol) resulted in a decrease of the permeability-surface area (PaS) product of phenylalanine of between 11 and 39%. In addition, the peptide elicited a decrease of the cerebral blood flow of between 11 and 56% combined with a drastic decrease of the cardiac output (32-64%) and an elevation of the blood pressure to approximately 150% of control values. However, we found no changes of the cardiac output, the blood pressure, or the PaS product of phenylalanine after microdialysis (30 min, 5 microliters min-1) of arginine vasopressin (15 mumol L-1) into the dorsal hippocampus, but cerebral blood flow was decreased. The results support the hypothesis that arginine vasopressin receptors at the blood-brain barrier are involved in the regulation of large neutral amino acid transfer from blood to brain and indicate that these receptors are located at the luminal membrane of the endothelial cells.
为了确定先前报道的血管加压素对亮氨酸血脑转运的影响是否也适用于其他大中性氨基酸，我们采用积分技术测量了L-苯丙氨酸的区域血脑转运。静脉联合注射L-苯丙氨酸和精氨酸血管加压素（30 nmol至10 pmol）导致苯丙氨酸的通透表面积（PaS）乘积降低了11%至39%。此外，该肽引起脑血流量降低了11%至56%，同时心输出量急剧下降（32% - 64%），血压升高至对照值的约150%。然而，在将精氨酸血管加压素（15 μmol L-1）微透析（30分钟，5微升/分钟）至背侧海马后，我们未发现心输出量、血压或苯丙氨酸的PaS乘积有变化，但脑血流量降低了。这些结果支持了血脑屏障处的精氨酸血管加压素受体参与调节大中性氨基酸从血液到脑的转运这一假说，并表明这些受体位于内皮细胞的管腔膜上。
