Mavziutov A R, Mirsaiapova I A, Baĭmiev A Kh, Khasanova G F, Mavziutova G A, Ochilova R A
Zh Mikrobiol Epidemiol Immunobiol. 2011 Nov-Dec(6):69-72.
Search of new targets and improvements of molecular-genetic detection methods of species specific DNA fragments of out-of-hospital pneumonia (OP) infection agents in sputum.
Species specific oligonucleotide primers to Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Branhamella catarrhalis were selected. 103 sputum samples from OP patients and 15 from practically healthy humans were test by using PCR and bacteriological method.
Mixed bacterial flora was detected by bacteriologic method in OP in 26 cases (25.2%). 14 patients (13.6%) had B. catarrhalis DNA in sputum, 12 patients (11.6%)--S. pneumoniae, H. influenzae was detected in 11 examined individuals (10.7%), K. pneumoniae (7.9%)-- in 8. In 32 cases (30%) etiology of OP could not be deciphered bacteriologically. B. catarrhalis was detected in 19 (18.5%) of OP patients, S. pneumoniae--in 17 (16.5%), H. influenzae and K. pneumoniae--in 14.5% and 12.6% (15 and 13 patients) respectively by Using the developed PCR primers. In 16.5% cases (17 patients) positive results against several bacteria species simultaneously were obtained by PCR method, in 3.9% (4 patients) P. aeruginosa was detected. CONCLUSION. Use of PCR for detection of out-of-hospital pneumonia agents DNA significantly increases informativity of the study when compared with bacteriological method.
寻找院外肺炎（OP）感染病原体痰液中物种特异性DNA片段的新靶点并改进分子遗传学检测方法。
选择针对肺炎链球菌、流感嗜血杆菌、肺炎克雷伯菌、铜绿假单胞菌、卡他布兰汉菌的物种特异性寡核苷酸引物。采用聚合酶链反应（PCR）和细菌学方法对103例OP患者的痰液样本和15例健康人的痰液样本进行检测。
细菌学方法检测出26例（25.2%）OP患者存在混合菌群。14例患者（13.6%）痰液中有卡他布兰汉菌DNA，12例患者（11.6%）有肺炎链球菌，11例受检者（10.7%）检测到流感嗜血杆菌，8例（7.9%）有肺炎克雷伯菌。32例（30%）OP患者的病因无法通过细菌学方法明确。使用开发的PCR引物，19例（18.5%）OP患者检测到卡他布兰汉菌，17例（16.5%）有肺炎链球菌，流感嗜血杆菌和肺炎克雷伯菌分别在14.5%（15例）和12.6%（13例）患者中检测到。PCR方法在16.5%（17例）的病例中同时获得了针对几种细菌的阳性结果，3.9%（4例）检测到铜绿假单胞菌。结论：与细菌学方法相比，使用PCR检测院外肺炎病原体DNA显著提高了研究的信息量。
