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[胡萝卜软腐欧文氏菌胡萝卜软腐亚种中长尾噬菌体科噬菌体的检测]

[Detection of bacteriophages of siphoviridae family in Erwinia carotovora subsp. carotovora].

作者信息

Ivanitsa T V, Tovkach F I

出版信息

Mikrobiol Z. 2011 Nov-Dec;73(6):57-63.

Abstract

It was established that the polylysogenic phage system of culture Erwinia carotovora subsp. carotovora 91P includes: a) defective bacteriophages of Myoviridae family, which are displayed as macromolecular carotovoricins b) valuable highly unstable temperate phage, which can be attributed to the family Myoviridae, and which, perhaps, is an analogue of phage ZF40 [6], and c) resistant to osmotic shock temperate phage of family Siphoviridae. This phage, called TIRI, consists of isometric head 50 nm in diameter and a rigid tail structure 203 nm long. A characteristic feature of the phage tail is an evident transverse striation, which is also indicative for the tail-like particle of the defective temperate phage of the strain 48A-7/4b. In general, the phage system of E carotovora subsp. carotovora is similar to Pseudomonas aeruginosa with its R- and F-bacteriocins, and phages of the families Myoviridae and Siphoviridae.

摘要

已确定胡萝卜软腐欧文氏菌胡萝卜软腐亚种91P培养物的多溶源噬菌体系统包括:a)肌尾噬菌体科的缺陷噬菌体,表现为大分子胡萝卜软腐菌素;b)有价值的高度不稳定的温和噬菌体,可归为肌尾噬菌体科,可能是噬菌体ZF40 [6]的类似物;c)对渗透压休克有抗性的长尾噬菌体科温和噬菌体。这种噬菌体称为TIRI,由直径50 nm的等面体头部和长203 nm的刚性尾部结构组成。噬菌体尾部的一个特征是明显的横向条纹,这也表明了菌株48A - 7/4b缺陷温和噬菌体的尾状颗粒。总体而言,胡萝卜软腐欧文氏菌胡萝卜软腐亚种的噬菌体系统与铜绿假单胞菌及其R - 型和F - 型细菌素以及肌尾噬菌体科和长尾噬菌体科的噬菌体相似。

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