Yale School of Public Health, 60 College Street, New Haven, CT 06520, USA.
J Med Entomol. 2012 Jan;49(1):77-84. doi: 10.1603/me11013.
The population genetic structure of Lutzomyia verrucarum (Townsend), a sand fly disease vector of Carrion's disease and cutaneous leishmaniasis in the Peruvian Andes, was characterized by sequencing 653 bp of cytochrome b and 1,125 bp of the NADH dehydrogenase subunit 4 genes of its mitochondrial genome. DNA sequence variation within and between valleys was compared in a sample of 220 sand flies from three valleys (Purisima, Huaylas, and Conchucos) and five departments (Amazonas, Cajamarca, Piura, Lima, and Huancavelica). Gene network and phylogenetic analyses indicated a high similarity of haplotypes collected within a single valley (0-0.52% nucleotide divergence). Flies from each valley had unique genotypes not shared with specimens from other valleys or from more distant regions (0.8-3.1% nucleotide divergence). Mountain ranges and geographic distance appear to have impeded migration (N(m) = < 0.18) between valleys and separated populations into discrete genetic units.
利用线粒体基因组细胞色素 b 基因 653 个碱基对和 NADH 脱氢酶亚单位 4 基因 1125 个碱基对对秘鲁安第斯山脉的致虻病和皮肤利什曼病的病媒虻属沙蝇 Lutzomyia verrucarum（汤森）的种群遗传结构进行了特征描述。在来自三个山谷（普里西马、瓦拉斯和孔科苏斯）和五个地区（亚马逊、卡哈马卡、皮乌拉、利马和万卡韦利卡）的 220 只沙蝇样本中比较了山谷内和山谷间的 DNA 序列变异。基因网络和系统发育分析表明，在单个山谷内收集的单倍型高度相似（核苷酸差异 0-0.52%）。来自每个山谷的蝇都具有独特的基因型，与来自其他山谷或更远地区的标本不共享（核苷酸差异 0.8-3.1%）。山脉和地理距离似乎阻碍了山谷之间（N(m) = <0.18）以及种群之间的迁徙，将种群分隔成离散的遗传单位。
