Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., PL-87-100 Toruń, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Mar 1;887-888:93-101. doi: 10.1016/j.jchromb.2012.01.017. Epub 2012 Jan 24.
The abnormal concentration of several modified nucleosides in the urine is supposed to be marker of carcinogenesis. For this reason analytical methods useful in the determination of these compounds in biological samples are of great importance. Present study concerns the application of Ultra High Performance Liquid Chromatography for fast, precise, high resolution separation and quantification of eight modified nucleosides in the synthetic serum and urine samples. The systematic study concerning the retention behavior of analyzed nucleosides on various stationary and mobile phases was performed. The attempt to apply four different column packings (octedecyl, octyl, phenyl, siliga gel) of different particle sizes and various mobile phases was made. On the basis of obtained results Kinetex C18 column and methanol/water mixtures were chosen for the utilization in biological samples. Developed method allows separation and quantification of eight modified nucleosides in serum or urine during 4min with good linearity, accuracy and low LOQ values.
尿液中几种修饰核苷的异常浓度被认为是致癌的标志物。因此，在生物样本中测定这些化合物的分析方法非常重要。本研究应用超高效液相色谱法对合成血清和尿液样品中的 8 种修饰核苷进行快速、精确、高分辨率的分离和定量。对分析核苷在各种固定相和流动相上的保留行为进行了系统研究。尝试使用四种不同粒径和不同流动相的不同填充柱（辛基、辛基、苯基、硅胶）。根据实验结果，选择 Kinetex C18 柱和甲醇/水混合物用于生物样品的分析。建立的方法可在 4 分钟内分离和定量血清或尿液中的 8 种修饰核苷，具有良好的线性、准确性和低的检出限。
