Laboratório de Doenças Parasitárias dos Animais Domésticos, Departamento de Medicina Veterinária -UFRPE, Recife, PE 52171-900, Brazil.
Vet Parasitol. 2012 Jul 6;187(3-4):548-52. doi: 10.1016/j.vetpar.2012.01.020. Epub 2012 Jan 18.
Bovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through polymerase chain reaction, genetic sequencing and bioinformatics analysis of the genes, a high degree of conservation (98-100%) was found among Brazilian isolates of B. bovis and the T2Bo isolate. Thus, these genes are worth considering as viable candidates to be included in a recombinant cocktail vaccine for cattle babesiosis caused by B. bovis.
牛巴贝斯虫病由巴贝斯虫引起，仍然是全球牛养殖业发展的重要制约因素。通过接种寄生虫的减毒活表型可以实现有效控制。然而，这些疫苗存在许多缺点，这也证明了需要寻找更好、更安全的疫苗。近年来，已经发现了许多具有免疫原性的寄生虫蛋白。然而，关于这些蛋白在不同寄生虫分离株中的遗传保守性的信息很少，这阻碍了它们作为免疫原的评估。本研究旨在评估 5 株巴西巴贝斯虫分离株中的 ama-1、acs-1、rap-1、trap、p0 和 msa2c 基因的保守性。通过聚合酶链反应、基因测序和生物信息学分析，发现巴西巴贝斯虫分离株和 T2Bo 分离株之间存在高度保守性（98-100%）。因此，这些基因值得考虑作为一种重组鸡尾酒疫苗的候选基因，用于预防由巴贝斯虫引起的牛巴贝斯虫病。
