Jones H, Steart P V, Du Boulay C E, Roche W R
Department of Pathology, Southhampton General Hospital, U.K.
J Pathol. 1990 Sep;162(1):29-33. doi: 10.1002/path.1711620107.
The immunoreactivity of a range of vascular and non-vascular smooth muscle tumours, rhabdomyosarcomas, and non-myoid lesions has been examined with the use of a monoclonal antibody to smooth muscle-specific actin and the muscle intermediate filament, desmin. In all cases of smooth muscle-derived tumours, the alpha-actin antibody yielded superior results. Staining of the myofibroblasts of fibromatoses was also seen. In contrast to desmin, immunoreactivity was not exhibited by rhabdomyosarcomas. We propose that this monoclonal antibody to alpha-smooth muscle actin is a useful addition to the panel of reagents used for the characterization of soft tissue proliferations and tumours. The technical aspects of the application of this monoclonal antibody to immunohistochemistry are discussed.
利用一种针对平滑肌特异性肌动蛋白和肌肉中间丝结蛋白的单克隆抗体，对一系列血管和非血管平滑肌肿瘤、横纹肌肉瘤及非肌样病变的免疫反应性进行了检测。在所有平滑肌源性肿瘤病例中，α-肌动蛋白抗体产生了更好的结果。纤维瘤病的肌成纤维细胞也可见染色。与结蛋白不同，横纹肌肉瘤未表现出免疫反应性。我们认为，这种针对α-平滑肌肌动蛋白的单克隆抗体是用于软组织增殖和肿瘤特征化的试剂组中的一项有用补充。本文讨论了将该单克隆抗体应用于免疫组织化学的技术方面。
