开发新型微卫星标记在峨眉树蛙（Rhacophorus omeimontis）。
Development of novel microsatellite markers in the Omei treefrog (Rhacophorus omeimontis).
机构信息
Animal Behaviour Research Group, College of Life Sciences, Central China Normal University, 152 Luoyulu, Hongshan District, Wuhan 430079, China.
出版信息
Int J Mol Sci. 2012;13(1):552-557. doi: 10.3390/ijms13010552. Epub 2012 Jan 5.
Eleven novel microsatellite markers were developed and characterized for the Omei treefrog (Rhacophorus omeimontis) using the fast isolation by AFLP of sequences containing repeats method. Polymorphism of each locus was tested in 24 individuals from two wild populations. The number of alleles per locus ranged from 4 to 15, the average observed and expected heterozygosity per locus ranged from 0.250 to 0.839 and from 0.562 to 0.914, respectively. Two of the 11 microsatellite loci showed significant deviations from Hardy-Weinberg equilibrium. Two locus pairs showed significant linkage disequilibrium. Neither evidence of scoring error due to stuttering nor evidence of large allele dropout was found at all of the 11 loci, but evidence of null alleles was indicated at two loci because of general excess of homozygotes for most allele size classes. These polymorphic loci will be useful markers in studying mate choice of the Omei treefrog.
利用扩增片段长度多态性（AFLP）测序重复序列快速分离的方法，为中国树蛙（Rhacophorus omeimontis）开发并鉴定了 11 个新的微卫星标记。在来自两个野生种群的 24 个个体中，对每个位点的多态性进行了测试。每个位点的等位基因数范围为 4 到 15，每个位点的观察杂合度和期望杂合度范围分别为 0.250 到 0.839 和 0.562 到 0.914。11 个微卫星位点中的 2 个位点偏离哈迪-温伯格平衡。有 2 对位点存在显著的连锁不平衡。在所有 11 个位点均未发现由于颤抖引起的计分错误或大片段缺失的证据，但由于大多数等位基因大小类别的同型合子数量普遍过多，有 2 个位点存在无效等位基因的迹象。这些多态性位点将成为研究中国树蛙配偶选择的有用标记。
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