Department of Neurosurgery, Qi Lu Hospital, Shandong University, Jinan, China.
Cancer Biol Ther. 2012 Apr;13(6):389-400. doi: 10.4161/cbt.19237. Epub 2012 Apr 1.
Sustaining proliferative signaling is one of the hallmarks of cancer. Granulocyte-colony stimulating factor (G-CSF), a hematopoietic growth factor that controls proliferation and differentiation of neural stem cells, may contribute to the development of glioma. In the present study, we evaluated the expression of G-CSF and its receptor (G-CSFR) in various grades of glioma samples and primary cell culture derived from a glioblastoma patient as well as different human glioma cell lines. We showed that G-CSF and G-CSFR were widely expressed by glioma samples, primary glioma cell culture and glioma cell lines. The expression levels of G-CSF and G-CSFR were not significantly different between different grades of glioma. G-CSF promoted proliferation, migration and invasion of glioma cells. Neutralization of G-CSFR with its antibody inhibited growth and metastasis of glioma cells in vitro. We also showed that activation of signal transducer and activator transcription 3 (STAT 3) as well as expression of several of its downstream effectors was regulated by G-CSF. Taken together, the present results suggest that G-CSF contributes to glioma progression that may be linked to glioma genesis and recurrence.
持续的增殖信号是癌症的特征之一。粒细胞集落刺激因子（G-CSF）是一种造血生长因子，可控制神经干细胞的增殖和分化，可能有助于神经胶质瘤的发生。在本研究中，我们评估了不同分级的神经胶质瘤样本、源自胶质母细胞瘤患者的原代细胞培养物以及不同的人神经胶质瘤细胞系中 G-CSF 及其受体（G-CSFR）的表达情况。结果显示，G-CSF 和 G-CSFR 广泛表达于神经胶质瘤样本、原代神经胶质瘤细胞培养物和神经胶质瘤细胞系中。不同分级的神经胶质瘤之间 G-CSF 和 G-CSFR 的表达水平无显著差异。G-CSF 可促进神经胶质瘤细胞的增殖、迁移和侵袭。用其抗体中和 G-CSFR 可抑制神经胶质瘤细胞在体外的生长和转移。我们还表明，G-CSF 可调节信号转导子和转录激活子 3（STAT3）的激活及其下游效应物的表达。综上所述，这些结果提示 G-CSF 有助于神经胶质瘤的进展，可能与神经胶质瘤的发生和复发有关。
