Pereira Jacintha Archana, Das Prosun, Chaklader Malay, Chatterjee Sumanta, Basak Pratima, Chaudhuri Samaresh, Law Sujata
Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata-700073, India.
J Stem Cells. 2010;5(3):117-27.
Apoptosis, proliferation and differentiation are balanced molecular processes which may alter their pattern during environmental insults. Arsenic is an environmental pollutant, ranks 20(th) in abundance in the earth crust, 14(th) in sea water and 12(th) in the human body. Millions of people worldwide are chronically exposed to arsenic often due to naturally occurring arsenic in ground water. Hematopoietic stem cells within the bone marrow are the source of all haematopoietic cell lineages and are essential for tissue development throughout the life. In this experimental study, we have evaluated the impact of arsenic, on blood and blood forming cells by the changes in their cellular morphology, immune functional capacity, alteration of bone marrow CD34 positive stem/progenitors and changes in the phenotype of Sca-1, c-Kit dual positive primitive stem cell population. The study revealed that arsenic has a significant effect on bone marrow and hematopoietic stem cells, their immune capacity and upregulation of death process, all indicative of impairment in differentiation suggesting presence of deregulation in their precursors by arsenic toxicity.
细胞凋亡、增殖和分化是平衡的分子过程，在环境损伤期间其模式可能会发生改变。砷是一种环境污染物，在地壳中的丰度排名第20，在海水中排名第14，在人体中排名第12。全世界数百万人经常因地下水中天然存在的砷而长期接触砷。骨髓中的造血干细胞是所有造血细胞谱系的来源，对整个生命过程中的组织发育至关重要。在本实验研究中，我们通过细胞形态学变化、免疫功能能力、骨髓CD34阳性干细胞/祖细胞的改变以及Sca-1、c-Kit双阳性原始干细胞群体表型的变化，评估了砷对血液和造血细胞的影响。研究表明，砷对骨髓和造血干细胞、它们的免疫能力以及死亡过程的上调有显著影响，所有这些都表明分化受损，提示砷毒性使其前体细胞存在失调。
