Kestler H W, Mori K, Silva D P, Kodama T, King N W, Daniel M D, Desrosiers R C
New England Regional Primate Research Center, Southborough, MA.
J Med Primatol. 1990;19(3-4):421-9.
Molecular clones of SIVmac were constructed that differed only in sequences within the nef gene. DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef. Rhesus monkeys that received each kind of molecularly cloned virus became infected. Our results additionally suggest that mutant forms of virus are selected in vitro while open, functional forms are selected in vivo. In animals infected with virus containing a stop codon within nef, reversion of the stop codon to a coding codon was demonstrated in five of five clones analyzed. These results indicate that nef is playing some role crucial to the virus life cycle in vivo.
构建了仅在nef基因序列上存在差异的SIVmac分子克隆。用含有开放阅读框的nef的病毒DNA进行DEAE转染，所产生的病毒在猕猴外周血淋巴细胞（PBL）培养物中的复制动力学和程度与nef基因内有缺失或终止密码子的病毒相似。接受每种分子克隆病毒的恒河猴都被感染了。我们的结果还表明，病毒的突变形式在体外被选择，而开放的、功能性形式在体内被选择。在感染了nef基因内含有终止密码子的病毒的动物中，在分析的五个克隆中有五个显示终止密码子回复为编码密码子。这些结果表明，nef在体内病毒生命周期中发挥着某些关键作用。
