Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Lab Chip. 2012 Mar 21;12(6):1151-6. doi: 10.1039/c2lc21096g. Epub 2012 Feb 9.
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
铜催化的叠氮-炔环加成（CuAAC）反应被应用于新型 DNA 在修饰的固体载体上的固定方法。CuAAC 点击反应能够使在其 5' 端修饰有戊炔基的 DNA 与负载叠氮化物的载玻片发生共价结合。采用这种方法制备了点击微阵列，并成功地应用于生物/模型实验。
