pH值和盐度对柱状黄杆菌和黏细菌在印度鲶鱼（胡子鲶，林奈）和印度囊鳃鲶（布洛赫）中的致病性的影响

Effect of pH and salinity on pathogenicity of Flavobacterium columnare and Myxobacterium sp. in Indian cat fish, Clarias batrachus (Linn.) and Heteropneustes fossilis (Bloch.).

作者信息

Verma Vinay, Prasad Yogendra, Singh Bhoj Raj

机构信息

Aquatic Biotechnology and Fish Pathology Laboratory, Department of Animal Science, M.J.P. Rohilkhand University, Bareilly - 243 006, India.

出版信息

J Environ Biol. 2011 Sep;32(5):573-7.

PMID:22319871

Abstract

Flavobacterium columnare (FC) and Myxobacterium sp. recorded persistently associated in fish hatchery and culture system of Himalayan and Sub - Himalayan regions were found to be pathogenic. The pH and salinity played a significant role on the pathogenicity of these potent pathogens in case of Clarias batrachus and Heteropneustes fossilis. LD50 value of FC was 10(4.5) CFU in both the fishes and those of Myxobacterium sp it was 10(6) CFU ml(-1) fish(-1). Fish challenged with F. columnare and Myxobacterium sp. (@ 0.2 ml fish(-1)) individually consisting 10(5-6) cfu ml(-1) exhibited explicit symptoms of columnaris disease and marked with ulceration and saddle back lesion on the dorsal side of body. Maximum reisolation of inoculated bacteria was recorded at pH 7.0 and 7.5 and at 0.0-0.5 (F. columnare) and 0.0-1.0% (Myxobacterium sp.) salinity. Foregoing results elucidated that F. columnare was more sensitive to salinity in comparison to Myxobacterium sp. and their pathogenicity significantly (p<0.05) depends on the salinity and pH that might be one of the physical factors to control their proliferation.

摘要

在喜马拉雅和次喜马拉雅地区的鱼类孵化场和养殖系统中持续发现的柱状黄杆菌（FC）和粘细菌与鱼类相关，且被发现具有致病性。在胡子鲶和印度胡鲶的案例中，pH值和盐度对这些强效病原体的致病性起着重要作用。FC在两种鱼类中的半数致死剂量（LD50）值为10⁽⁴·⁵⁾CFU，而粘细菌的LD50值为10⁽⁶⁾CFU ml⁻¹ 鱼⁻¹。用柱状黄杆菌和粘细菌（@0.2 ml鱼⁻¹）分别以10⁽⁵⁻⁶⁾ cfu ml⁻¹的浓度对鱼进行攻击，鱼表现出明显的柱状病症状，身体背部有溃疡和鞍背病变。在pH值为7.0和7.5以及盐度为0.0 - 0.5（柱状黄杆菌）和0.0 - 1.0%（粘细菌）时，接种细菌的再分离率最高。上述结果表明，与粘细菌相比，柱状黄杆菌对盐度更敏感，并且它们的致病性显著（p<0.05）取决于盐度和pH值，这可能是控制它们增殖的物理因素之一。