Ikuse Tamaki, Ohtsuka Yoshikazu, Kudo Takahiro, Hosoi Kenji, Obayashi Naho, Jimbo Keisuke, Aoyagi Yo, Fujii Tohru, Nagata Satoru, Shimizu Toshiaki
Department of Pediatric and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Pediatr Int. 2012 Jun;54(3):319-24. doi: 10.1111/j.1442-200X.2012.03573.x. Epub 2012 Apr 18.
Although initial infection with Helicobacter pylori may occur before 5 years of age, the pediatric mucosal immune response against H. pylori is not clear. The aim of the present study was to evaluate immune responses in the H. pylori-infected gastric mucosa of children using microarray and real-time polymerase chain reaction (PCR) analysis of pediatric gastric samples.
Gastric samples were obtained from 12 patients undergoing routine endoscopy of chronic abdominal complaints. Six patients (three boys, three girls) aged 10.1-14.6 years had evidence of H. pylori infection, and the remaining six (three boys, three girls) aged 10.3-15.5 years had no evidence of infection and presented no histological changes associated with gastritis. Microarray and real-time PCR analyses were performed, and the changes in gene expression-related immune response were also analyzed.
Using microarray analysis, the total number of significantly upregulated and downregulated genes (fold change >5, P < 0.01) was 21 in the antrum and 16 in the corpus when comparing patients with or without infection. Using real-time PCR, the expression of lipocalin-2 (Lcn2), C-C motif chemokine ligand (CCL) 18, C-X-C motif chemokine ligand (CXCL) 9 and CXCL11 was upregulated, while the expression of pepsinogen (PG) I and PGII was downregulated when comparing patients with or without infection.
Lcn2, CCL18, CXCL9, CXCL11, PGI and PGII play important roles in childhood H. pylori infection.
尽管幽门螺杆菌的初次感染可能发生在5岁之前，但儿童针对幽门螺杆菌的黏膜免疫反应尚不清楚。本研究的目的是通过对儿童胃样本进行微阵列和实时聚合酶链反应（PCR）分析，评估幽门螺杆菌感染的儿童胃黏膜中的免疫反应。
从12例因慢性腹部不适接受常规内镜检查的患者中获取胃样本。6例年龄在10.1 - 14.6岁的患者（3名男孩，3名女孩）有幽门螺杆菌感染的证据，其余6例年龄在10.3 - 15.5岁的患者（3名男孩，3名女孩）没有感染证据，且未出现与胃炎相关的组织学变化。进行了微阵列和实时PCR分析，并分析了基因表达相关免疫反应的变化。
通过微阵列分析，比较感染组和未感染组患者时，胃窦中显著上调和下调的基因总数（倍数变化>5，P < 0.01）为21个，胃体中为16个。通过实时PCR，比较感染组和未感染组患者时，脂质运载蛋白2（Lcn2）、C - C基序趋化因子配体（CCL）18、C - X - C基序趋化因子配体（CXCL）9和CXCL11的表达上调，但胃蛋白酶原（PG）I和PGII的表达下调。
Lcn2、CCL18、CXCL9、CXCL11、PGI和PGII在儿童幽门螺杆菌感染中起重要作用。
