Departamento de Ingeniería Química y Ambiental, Universidad Técnica Federico Santa María, Valparaíso, Chile.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(3):420-7. doi: 10.1080/10934529.2012.646129.
An evaluation of natural zeolite as a microorganism carrier in nitrifying reactors operated in batch mode was carried out. Specifically, the influence of zeolite particle sizes of 0.5, 1.0 and 2.0 mm in diameter on microorganism adherence to zeolite, ammonium adsorption capacity and the identification of microbial populations were assessed. The greatest amount of total biomass adhered was observed for a zeolite particle size of 1 mm (0.289 g) which was achieved on the 12th day of operation. The highest ammonium adsorption capacity was observed for a zeolite particle size of 0.5 mm, which was 64% and 31% higher than that observed for particle sizes of 1.0 and 2.0 mm, respectively. The maximum de-sorption values were also found for a zeolite particle size of 0.5 mm, although when equilibrium was reached the ammonium concentrations were similar to those observed for a zeolite particle size of 1.0 mm. It was also found that the experimental data on ammonium adsorption fitted very well to the Freundlich isotherm for the three particle sizes studied. Finally, the nitrifying reactors showed similar microbial populations independently of the particle size used as microorganism carrier. The dominant bacterial community was Gammaproteobacteria making up 80% of the total population found. Betaproteobacteria were also identified and made up 12% approx. of the total population. Ammonium Oxidant Betaproteobacteria and Nitrobacter were also detected.
评价沸石作为微生物载体在间歇式硝化反应器中的应用。具体来说，评估了直径为 0.5、1.0 和 2.0 毫米的沸石颗粒对微生物在沸石上的附着、氨氮吸附能力和微生物种群鉴定的影响。在运行的第 12 天，观察到 1 毫米粒径的沸石（0.289g）上附着的总生物量最多。对于 0.5 毫米粒径的沸石，氨氮吸附容量最高，比 1.0 毫米和 2.0 毫米粒径的沸石分别高 64%和 31%。0.5 毫米粒径的沸石也具有最大的解吸值，但达到平衡时，氨氮浓度与 1.0 毫米粒径的沸石相似。还发现，对于所研究的三种粒径，氨氮吸附的实验数据非常符合 Freundlich 等温线。最后，硝化反应器显示出相似的微生物种群，而不依赖于用作微生物载体的粒径。优势细菌群落是γ变形菌，占总种群的 80%。还鉴定出β变形菌，占总种群的 12%左右。还检测到氨氧化β变形菌和硝化菌。
