Wnt/LRP5/β-连环蛋白信号通路对绝经后骨质疏松症发病机制的影响

[Effect of the Wnt/LRP5/β-catenin signaling pathway on the pathogenesis of postmenopausal osteoporosis].

作者信息

Wang Yan, Liu Yan, Ma Jian-xia, Li Bao-xin, Li Yu-kun

机构信息

Department of Endocrinology, Third Hospital of Hebei Medical University, Shijiazhuang 050051, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2011 Oct;46(10):769-72.

PMID:22321352

Abstract

OBJECTIVE

To investigate the role of the Wnt/LRP5/β-catenin signaling pathway in the pathogenesis of postmenopausal osteoporosis.

METHODS

Fifty female Wistar rats aged 6-month-old, were randomly divided into control group (NS, n = 24) and ovariectomized group (NOVX, n = 26), NOVX underwent bilateral ovariectomy. At 0, 4 and 8 weeks, all of rats were measured blood estrogen (E(2)) and bone mineral density (BMD), 4 and 8 weeks, low density lipoprotein receptor-related protein 5 (LRP5), β-catenin and Runx2 mRNA in bone were measured respectively by reverse transcription (RT)-PCR.

RESULTS

In 4 and 8 weeks, compared with NS which had (117 ± 29) and (114 ± 15) pmol/L in E(2) level, (0.098 ± 0.016) and (0.095 ± 0.028) g/cm(2) in BMD, NOVX had significantly decreased to (92 ± 15) and (95 ± 22) pmol/L in E(2) level (P < 0.05), (0.076 ± 0.016) and (0.052 ± 0.013) g/cm(2) in BMD values (P < 0.01). And bone tissue LRP5, β-catenin and Runx2 mRNA expression was 1.02 ± 0.06, 1.04 ± 0.05, 1.07 ± 0.21 in NS, NOVX was significantly reduced to 0.97 ± 0.04, 0.58 ± 0.05, 0.86 ± 0.03 (P < 0.05).

CONCLUSION

Wnt/LRP5/β-catenin signaling pathway may be important in the pathogenesis of postmenopausal osteoporosis.

摘要

目的

探讨Wnt/LRP5/β-连环蛋白信号通路在绝经后骨质疏松症发病机制中的作用。

方法

将50只6月龄雌性Wistar大鼠随机分为对照组（NS，n = 24）和去卵巢组（NOVX，n = 26），对NOVX组进行双侧卵巢切除术。在0、4和8周时，检测所有大鼠的血雌激素（E₂）和骨密度（BMD），在4和8周时，分别通过逆转录（RT）-PCR检测骨组织中低密度脂蛋白受体相关蛋白5（LRP5）、β-连环蛋白和Runx2 mRNA的表达。

结果

在4周和8周时，与E₂水平分别为（117±29）和（114±15）pmol/L、BMD分别为（0.098±0.016）和（0.095±0.028）g/cm²的NS组相比，NOVX组的E₂水平显著降低至（92±15）和（95±22）pmol/L（P<0.05），BMD值分别为（0.076±0.016）和（0.052±0.013）g/cm²（P<0.01）。并且骨组织中LRP5、β-连环蛋白和Runx2 mRNA的表达在NS组分别为1.02±0.06、1.04±0.05、1.07±0.21，在NOVX组显著降低至0.97±0.04、0.58±0.05、0.86±0.03（P<0.05）。