Zhang Yongqing, Liu Mingming, Li Haisheng, Chen Zetao, Liang Na, Xu Jianguo, Zhang Xiaoli, Zhang Yihang
Department of Orthopedics, Shouguang Traditional Chinese Medicine Hospital, Shouguang, China.
Department of Endocrinology, Shouguang Traditional Chinese Medicine Hospital, Shouguang, China.
Int J Rheum Dis. 2017 Aug;20(8):941-948. doi: 10.1111/1756-185X.13050. Epub 2017 Mar 13.
The aim of this study was to evaluate the therapeutic effect of a traditional Chinese medicine (TCM), Bushen-Jianpi-Huoxue decoction (BJHD), on diabetic osteoporosis (DOP) and the action mechanisms likely mediated by nuclear factor-kappa B (NF-κB) and Wnt signaling pathways.
Fifty-five male Wistar rats were used in this study; they were divided into normal control (n = 10) and established DOP model (n = 45) groups. The DOP model was induced using a combination high carbohydrate - high fat diet and intraperitoneal injections of streptozotocin (STZ). The successfully induced animals were randomized to the model, Western medicine, TCM and control groups. Levels of fasting blood glucose; insulin; serum Ca, P and alkaline phosphatase, and the femoral bone mineral density (BMD) were measured. Furthermore, messenger RNA (mRNA) levels of cytokines in the Wnt and NF-κB signaling pathways were measured using reverse transcription-polymerase chain reaction (RT-PCR).
Thirty rats were successfully established as the DOP model (10/group). After treatment, the levels of fasting blood glucose, insulin resistance and alkaline phosphatase in the TCM group rats were lower, while P and BMD were higher than those in the model groups. The mRNA levels of cytokines in the Wnt signaling pathway were higher in the TCM group than those in the model group. Moreover, the expressions of factors in the NF-κB pathway were markedly lower in the TCM group than they were in the model group.
Bushen-Jianpi-Huoxue decoction relieved DOP by activating the Wnt signaling pathway while inhibiting NF-κB signaling.
本研究旨在评估中药补肾健脾活血汤（BJHD）对糖尿病性骨质疏松症（DOP）的治疗效果以及可能由核因子-κB（NF-κB）和Wnt信号通路介导的作用机制。
本研究使用了55只雄性Wistar大鼠；将它们分为正常对照组（n = 10）和建立的DOP模型组（n = 45）。采用高碳水化合物-高脂肪饮食联合腹腔注射链脲佐菌素（STZ）诱导DOP模型。将成功诱导的动物随机分为模型组、西药组、中药组和对照组。测量空腹血糖、胰岛素、血清钙、磷和碱性磷酸酶水平以及股骨骨密度（BMD）。此外，使用逆转录-聚合酶链反应（RT-PCR）测量Wnt和NF-κB信号通路中细胞因子的信使核糖核酸（mRNA）水平。
成功建立了30只大鼠的DOP模型（每组10只）。治疗后，中药组大鼠的空腹血糖、胰岛素抵抗和碱性磷酸酶水平较低，而磷和BMD高于模型组。中药组Wnt信号通路中细胞因子的mRNA水平高于模型组。此外，中药组NF-κB通路中因子的表达明显低于模型组。
补肾健脾活血汤通过激活Wnt信号通路同时抑制NF-κB信号通路来缓解DOP。
