采用斑点酶联免疫吸附测定法对血迹进行ABO血型鉴定。
ABO blood-typing of bloodstains by dot-ELISA method.
作者信息
Ito S, Matsui K, Suzuki K, Matsuo Y, Matsumoto H
机构信息
Department of Legal Medicine, Osaka Medical College, Takatsuki, Japan.
出版信息
Nihon Hoigaku Zasshi. 1990 Jun;44(3):199-204.
The ABH antigens of bloodstains which were effectively solubilized in phosphate-buffered saline by crushing in a mortar were detected on a nitrocellulose membrane by enzyme-linked immunosorbent assay (ELISA). The analyses for characterization showed that the extracted substances consisted of various components of the red cell with molecular weights of more than 30,000. ABO types of some aged bloodstains were determined by this simple procedure. This method is a useful tool for determining various blood-groups from bloodstain.
通过在研钵中研磨而有效溶解于磷酸盐缓冲盐溶液中的血迹ABH抗原，采用酶联免疫吸附测定（ELISA）在硝酸纤维素膜上进行检测。特性分析表明，提取的物质由分子量超过30,000的红细胞的各种成分组成。通过这个简单的程序可以确定一些陈旧血迹的ABO血型。该方法是从血迹中确定各种血型的有用工具。
Zotero 插件