Division of Allergy-Immunology-Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.
Clin Exp Rheumatol. 2012 Mar-Apr;30(2):171-7. Epub 2012 Apr 13.
Decoy receptor 3 (DCR3) was a newly identified soluble receptor which was reported to modulate the function of T cells, dendritic cells and macrophages. The aim of this study was to investigate DCR3 expression on the synovial tissue in different types of arthritis.
We obtained synovial tissues from 17 rheumatoid arthritis (RA), 17 ankylosing spondylitis (AS) and 17 osteoarthritis (OA) patients. Synovial specimens were stained with hematoxylin and eosin. The amount of lymphocytes and mononuclear cells infiltration and vascularity during light microscopic examination was scored from 0-4. The expression of CD3, CD4, CD8, CD68 and DCR3 in lining layer (LL) and sublining layer (SL) cells was stained using the immunohistochemical method and analysed by microscopic examination (score from 0-4, 0=absent, 1=slight, 2=moderate, 3=large, 4=extreme).
OA patients were older than the RA and AS patients (65.9±10.3 years for OA, 58.4±17.7 for RA, and 43.2±16.4 for AS). Synovial tissues in RA patients had significantly increased mononuclear cells infiltration when compared to AS and OA patients (2.3±0.6, 1.9±0.5, 1.6±0.5, respectively, p<0.05). There was no striking difference in DCR3 expression in the synovial LL between RA, AS, and OA patients. CD4+ T cells and CD68+ monocytes/macrophages in the SL were more prominent in RA and AS than in OA (p<0.05). Similarly, DCR3 in the SL was more overexpressed in RA and AS than in OA (1.83±0.21, 1.71±0.36, 1.39±0.31, respectively, p<0.01).
The increased synovial inflammatory cells infiltration in RA and AS was associated with the elevated DCR3 expression.
诱饵受体 3（DCR3）是一种新发现的可溶性受体，据报道它可以调节 T 细胞、树突状细胞和巨噬细胞的功能。本研究旨在探讨不同类型关节炎滑膜组织中 DCR3 的表达。
我们从 17 例类风湿关节炎（RA）、17 例强直性脊柱炎（AS）和 17 例骨关节炎（OA）患者中获得滑膜组织。滑膜标本用苏木精和伊红染色。在光镜下，淋巴细胞和单核细胞浸润的数量以及血管生成的程度评分范围为 0-4 分。采用免疫组织化学方法检测衬里层（LL）和下衬里层（SL）细胞中 CD3、CD4、CD8、CD68 和 DCR3 的表达，并通过显微镜检查进行分析（评分范围为 0-4 分，0=无，1=轻微，2=中度，3=大，4=极度）。
OA 患者比 RA 和 AS 患者年龄更大（OA 患者为 65.9±10.3 岁，RA 患者为 58.4±17.7 岁，AS 患者为 43.2±16.4 岁）。与 AS 和 OA 患者相比，RA 患者滑膜组织中的单核细胞浸润明显增加（分别为 2.3±0.6、1.9±0.5、1.6±0.5，p<0.05）。RA、AS 和 OA 患者滑膜 LL 中 DCR3 的表达无明显差异。RA 和 AS 患者 SL 中的 CD4+T 细胞和 CD68+单核细胞/巨噬细胞比 OA 患者更明显（p<0.05）。同样，RA 和 AS 患者 SL 中的 DCR3 表达也高于 OA 患者（分别为 1.83±0.21、1.71±0.36、1.39±0.31，p<0.01）。
RA 和 AS 中滑膜炎症细胞浸润增加与 DCR3 表达升高有关。
