Department of Animal Resources, College of Agriculture, University of Baghdad, Iraq.
Anim Reprod Sci. 2012 Jan;130(1-2):33-41. doi: 10.1016/j.anireprosci.2012.01.002. Epub 2012 Jan 23.
This study was carried out to demonstrate the pregnancy-specific protein B (PSPB), progesterone and some biochemical parameters concentrations in amniotic fluid, allantoic fluid and fetal serum collected from slaughtered Iraqi riverine pregnant buffaloes at three different months of gestation (6th, 7th and 8th). Ten out of 22 adult buffaloes of 4.6 ± 0.97 years old were used in this study. The buffaloes were mated naturally by monitoring the estrus cycles via appearance of vaginal fluids and mounting by bulls. Pregnancy was checked for these buffaloes by non-returning to estrus for three estrus cycles and assured by rectal palpation on day 61 post-mating (PM). Buffaloes were slaughtered at three different periods of gestation (three at 6th month, four at 7th month and three at 8th month of gestation) to verify the progesterone and PSPB as well as some blood attributes levels (glucose, cholesterol, total protein, albumin, globulins and albumin: globulins ratio) in amniotic fluid (AF), allantoic fluid (LF) and fetal serum (FS). Progesterone was higher (P<0.01) in LF at the 8th month of gestation and lower in FS during the 7th and 8th months of pregnancy. PSPB concentrations were greater in FS (6th and 8th months in particular) than in both AF and LF. The overall mean of cholesterol concentration was higher in FS (P<0.05) followed by AF and LF that had the lowest concentration. The FS exhibited higher total protein during the three gestation periods. Most of fetal and placental measurements increased as the pregnancy advanced. In conclusion, these results described, for the first time, the PSPB and progesterone concentrations and blood characteristics in fetal fluids and serum in water riverine buffaloes during different stages of pregnancy. Progesterone concentrations were greater in allantoic fluid than in other fluids. In contrast, PSPB and other blood attributes were higher in fetal serum than other fluids of Iraqi riverine buffaloes. These findings reflect the changes in hormones, proteins and other metabolites during different gestation periods.
本研究旨在展示从屠宰的伊拉克河流妊娠水牛的羊水、尿囊液和胎儿血清中收集的妊娠特异性蛋白 B (PSPB)、孕酮和一些生化参数浓度，这些水牛在妊娠的三个不同月份（第 6、7 和 8 个月）进行了采集。本研究使用了 22 头 4.6±0.97 岁的成年水牛中的 10 头。这些水牛通过观察阴道分泌物和公牛的交配行为来监测发情周期，自然交配。通过三个发情周期不发情来检查这些水牛是否怀孕，并在交配后第 61 天通过直肠触诊来确认（PM）。水牛在三个不同的妊娠阶段（6 个月时 3 头，7 个月时 4 头，8 个月时 3 头）被屠宰，以验证孕酮和 PSPB 以及羊水 (AF)、尿囊液 (LF) 和胎儿血清 (FS) 中的一些血液属性水平（葡萄糖、胆固醇、总蛋白、白蛋白、球蛋白和白蛋白：球蛋白比值）。在第 8 个月的妊娠中，LF 中的孕酮水平更高（P<0.01），而在第 7 和第 8 个月的妊娠中，FS 中的孕酮水平更低。PSPB 浓度在 FS（特别是第 6 和第 8 个月）中高于 AF 和 LF。胆固醇浓度的总体平均值在 FS（P<0.05）中较高，其次是 AF 和 LF，它们的浓度最低。FS 在三个妊娠期间表现出较高的总蛋白。随着妊娠的进展，大多数胎儿和胎盘测量值增加。总之，这些结果首次描述了在不同妊娠阶段，水河流域水牛胎儿液和血清中的 PSPB 和孕酮浓度以及血液特征。在所有液体中，孕酮浓度在尿囊液中最高。相反，PSPB 和其他血液属性在胎儿血清中高于伊拉克河流域水牛的其他液体。这些发现反映了不同妊娠阶段激素、蛋白质和其他代谢物的变化。
