Université Paris-Sud, School of Pharmacy, UMR CNRS, Châtenay Malabry, France.
Drug Discov Today. 2012 Jun;17(11-12):623-9. doi: 10.1016/j.drudis.2012.01.023. Epub 2012 Feb 3.
Isothermal titration calorimetry (ITC) is one of the most interesting methods for the characterization of the interaction mechanisms of cyclodextrins (CDs) with drugs. In this review we explain how to conduct ITC experiments correctly for CD-guest interactions, how to choose an accurate fitting model for the titration curve and how to interpret carefully the ITC results. Finally, the use of ITC for the characterization of CD-containing nanoparticles is discussed.
等温滴定量热法（ITC）是研究环糊精（CDs）与药物相互作用机制的最有趣的方法之一。在这篇综述中，我们解释了如何正确进行 CD-客体相互作用的 ITC 实验，如何为滴定曲线选择准确的拟合模型，以及如何仔细解释 ITC 结果。最后，讨论了 ITC 在含 CD 纳米粒子表征中的应用。
