Chongqing University of Posts and Telecommunications, Chongqing, People's Republic of China.
Gene. 2012 Apr 15;497(2):164-71. doi: 10.1016/j.gene.2012.01.064. Epub 2012 Feb 2.
NTE-related esterase (NRE) is an insulin-regulated lysophospholipase with homology to neuropathy target esterase (NTE), which plays a role in energy metabolism. Here, we reported two alternative splicing variants of the murine NRE (mNRE) gene, termed mNREV1 and mNREV2. Genomic organization analysis indicated that 5' splice site of mNRE intron 33 was changed in both mNREV1 and mNREV2, and mNRE exon 21 was deleted in mNREV2. mNREV1 had the same protein domains with mNRE, while mNREV2 lacked the patatin domain in the C-terminal catalytic region. Green fluorescent protein-mNREV1 or mNREV2 fusion proteins localized to the endoplasmic reticulum. mNREV1 and mNRE exhibited equal hydrolytic activity to the substrate phenyl valerate, whereas mNREV2 did not have any catalytic activity. The expression profiles of mNRE and its splicing isoforms in white adipose tissue, cardiac muscle, skeletal muscle, and testis tissues were further analyzed by real time quantitative-PCR in fed and fasted states, which indicated that the major isoform of mNRE mRNA generated switched from mNREV2 to mNREV1 during fasting. Thus there was a nutritional regulation of mNRE expression at the mRNA levels via alternative splicing.
NTE 相关酯酶（NRE）是一种胰岛素调节的溶血磷脂酶，与神经病变靶酯酶（NTE）具有同源性，在能量代谢中发挥作用。在这里，我们报道了小鼠 NRE（mNRE）基因的两种选择性剪接变体，分别称为 mNREV1 和 mNREV2。基因组组织分析表明，mNREV1 和 mNREV2 中第 33 号内含子的 5'剪接位点均发生了改变，并且 mNREV2 缺失了外显子 21。mNREV1 与 mNRE 具有相同的蛋白结构域，而 mNREV2 在 C 端催化区缺失了 patatin 结构域。绿色荧光蛋白-mNREV1 或 mNREV2 融合蛋白定位于内质网。mNREV1 和 mNRE 对苯戊酸底物具有相同的水解活性，而 mNREV2 则没有任何催化活性。通过实时定量 PCR 在进食和禁食状态下进一步分析了 mNRE 及其剪接异构体在白色脂肪组织、心肌、骨骼肌和睾丸组织中的表达谱，结果表明，在禁食过程中，mNRE mRNA 的主要剪接异构体从 mNREV2 切换为 mNREV1。因此，NRE 的表达在 mRNA 水平上存在营养调节的选择性剪接。
