Department of Chemistry, Istanbul University, Avcilar, 34320 Istanbul, Turkey.
Anal Biochem. 2012 Apr 1;423(1):36-8. doi: 10.1016/j.ab.2012.01.011. Epub 2012 Jan 26.
The cupric reducing antioxidant capacity (CUPRAC) method using bathocuproine sulfonate (BCS) as cuprous-chelating ligand claims to be an efficient substitute for the conventional CUPRAC assay using neocuproine; however, differences in their physical chemistry give rise to significantly varying responsivities to antioxidant compounds. The antioxidant capacity due to β-carotene (in acetone or dichloromethane) was fully measurable by conventional CUPRAC but not at all by cupric-BCS assay. Furthermore, CUPRAC exhibits distinctly stronger responses than cupric-BCS to naringenin, α-tocopherol, and bilirubin.
采用浴铜灵（BCS）作为铜配合物配体的铜还原抗氧化能力（CUPRAC）方法声称是替代传统使用邻二氮菲的 CUPRAC 测定法的有效方法；然而，它们在物理化学性质上的差异导致对抗氧化化合物的响应性有显著差异。β-胡萝卜素（在丙酮或二氯甲烷中）的抗氧化能力可以通过传统的 CUPRAC 完全测量，但不能通过铜-BCS 测定法进行测量。此外，CUPRAC 对柚皮素、α-生育酚和胆红素的响应明显强于铜-BCS。
