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透过薄混浊介质的三维扫描显微镜术。

Three-dimensional scanning microscopy through thin turbid media.

作者信息

Yang Xin, Hsieh Chia-Lung, Pu Ye, Psaltis Demetri

机构信息

Optics Laboratory, Ecole Polytechnique Frederale de Lausanne (EPFL), Lausanne, 1015, Switzerland.

出版信息

Opt Express. 2012 Jan 30;20(3):2500-6. doi: 10.1364/OE.20.002500.

Abstract

We demonstrate three-dimensional imaging through a thin turbid medium using digital phase conjugation of the second harmonic signal emitted from a beacon nanoparticle. The digitally phase-conjugated focus scans the volume in the vicinity of its initial position through numerically manipulated phase patterns projected onto the spatial light modulator. Accurate three dimensional images of a fluorescent sample placed behind a turbid medium are obtained.

摘要

我们利用信标纳米粒子发射的二次谐波信号的数字相位共轭,展示了通过薄浑浊介质的三维成像。数字相位共轭焦点通过投影到空间光调制器上的数值操纵相位图案,在其初始位置附近扫描体积。获得了放置在浑浊介质后面的荧光样品的精确三维图像。

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