局域调制显微镜术

Focal modulation microscopy.

作者信息

Chen Nanguang, Wong Chee-Howe, Sheppard Colin J R

机构信息

Division of Bioengineering, National University of Singapore, Singapore.

出版信息

Opt Express. 2008 Nov 10;16(23):18764-9. doi: 10.1364/oe.16.018764.

DOI:10.1364/oe.16.018764

PMID:19581963

Abstract

We report a novel light microscopy method for high resolution molecular imaging of thick biological tissues with one photon excited fluorescence. Effective optical sectioning and diffraction limited spatial resolution are achieved when imaging deep inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by scattered light. Our method has been validated with animal tissue and an imaging depth around 600 microns has been demonstrated.

摘要

我们报告了一种用于厚生物组织高分辨率分子成像的新型光学显微镜方法，该方法采用单光子激发荧光。当通过使用焦点调制（一种抑制散射光激发的背景荧光信号的技术）对多散射介质内部深处进行成像时，可实现有效的光学切片和衍射极限空间分辨率。我们的方法已在动物组织上得到验证，并已证明成像深度约为600微米。