Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Bioresour Technol. 2012 Apr;110:224-31. doi: 10.1016/j.biortech.2012.01.088. Epub 2012 Jan 28.
This study investigated the preparation and characteristics of protein-coated microcrystals (PCMCs) from Pseudomonas cepacia lipase (PS) and K(2)SO(4), and their application in biodiesel synthesis, via single factorial experiments and response surface methodology (RSM), the optimized PCMC-PS exhibited high activity and stability; the optimal temperature was 60°C (which gave 99.83% conversion), although fairly high activity was exhibited after incubation at different temperatures (25-70°C). The organic solvents stability of the PCMC-PS was improved, and it significantly reduced ethanol toxicity. Circular dichroism (CD) analysis revealed the correlation between the conformation and the enzyme activity. The morphology of the PCMC-PS was also confirmed via scanning electron microscopy (SEM). When catalyzed by PCMC-PS, above 83% biodiesel yield was obtained for most of the seven oils tested. The PCMC-PS (washed with hexane) activity remained relatively stable after eight batch reactions, with only a 15.73% reduction in the conversion (from 99.02% to 83.29%).
本研究通过单因素实验和响应面法（RSM）考察了假单胞菌脂肪酶（PS）和 K2SO4制备蛋白包埋微晶体（PCMCs）的条件和特性，并将其应用于生物柴油合成。优化后的 PCMC-PS 表现出高活性和稳定性；最佳温度为 60°C（转化率为 99.83%），尽管在不同温度（25-70°C）下孵育后仍表现出相当高的活性。PCMC-PS 的有机溶剂稳定性得到提高，显著降低了乙醇毒性。圆二色性（CD）分析揭示了构象与酶活性之间的相关性。通过扫描电子显微镜（SEM）也证实了 PCMC-PS 的形态。当用 PCMC-PS 催化时，在所测试的七种油中，超过 83%的生物柴油产率。用正己烷洗涤后的 PCMC-PS 在八批反应后活性仍相对稳定，转化率仅降低了 15.73%（从 99.02%降至 83.29%）。
