Bachmann J, Schlüter H, Storkebaum W, Witzel H, Zidek W
Medizinische Poliklinik, Universität Münster, West Germany.
Life Sci. 1990;47(15):1365-74. doi: 10.1016/0024-3205(90)90201-2.
Different vascular models of normotensive Wistar rats, including aortic strips, isolated perfused mesentery and isolated perfused kidney, were used to study hemodynamic effects of plasma fractions obtained by gel filtration from the blood of essential hypertensive and normotensive subjects. Plasma fractions from essential hypertensives studied had been shown to increase blood pressure after intravenous injection in rats. In the aortic strips, 50 microliters of a hypertensive fraction (HF) elicited a calcium-dependent contraction of 0.14 +/- 0.035 mN (n = 20, p less than 0.05), which was inhibited by nifedipine, whereas tension of the strips was not significantly changed by normotensive fractions (NF) (n = 17). In the isolated perfused mesentery preparation, no significant change of perfusion pressure by HF or NF could be demonstrated (n = 10). In the isolated perfused kidney, a transient increase of perfusion pressure was induced by HF (19.5 +/- 16.6 mm Hg, n = 40, P less than 0.001) but not by NF. This increase was abolished in calcium-free, 2 mmol/l EGTA containing perfusion medium. The response was diminished, but not abolished by nifedipine. These data demonstrate vasopressor properties of plasma from essential hypertensives, which might be the consequence of a circulating vasoconstrictor substance in the blood of essential hypertensives.
利用正常血压的Wistar大鼠的不同血管模型，包括主动脉条、离体灌注肠系膜和离体灌注肾脏，来研究通过凝胶过滤从原发性高血压患者和正常血压患者血液中获得的血浆组分的血流动力学效应。研究表明，原发性高血压患者的血浆组分在大鼠静脉注射后会使血压升高。在主动脉条实验中，50微升高血压组分（HF）引起了0.14±0.035毫牛顿的钙依赖性收缩（n = 20，p < 0.05），硝苯地平可抑制该收缩，而正常血压组分（NF）对主动脉条张力无显著影响（n = 17）。在离体灌注肠系膜制备实验中，未观察到HF或NF对灌注压力有显著变化（n = 10）。在离体灌注肾脏实验中，HF可引起灌注压力短暂升高（19.5±16.6毫米汞柱，n = 40，P < 0.001），而NF则无此作用。在不含钙、含有2毫摩尔/升乙二醇双四乙酸（EGTA）的灌注介质中，这种升高被消除。硝苯地平可使该反应减弱，但未消除。这些数据表明原发性高血压患者血浆具有升压特性，这可能是原发性高血压患者血液中循环血管收缩物质作用的结果。
