Department of Pharmacology, University of Melbourne, Melbourne, Victoria, Australia.
Stem Cells. 2012 May;30(5):811-6. doi: 10.1002/stem.1055.
Despite burgeoning interest in the potential of cellular therapies in lung regenerative medicine, progress in delivering these therapies has been confounded by a lack of knowledge about the identity of appropriate targets which can be harnessed to repair the lung, and the cellular and molecular factors which regulate their regenerative potential. While systematic analysis of lung development and cell lineage tracing studies in normal and perturbed animal models provides a framework for understanding the complex interplay of the multiple cell types, biomatrix elements and soluble and insoluble cytokines and factors that regulate lung structure and function, a reductionist approach is also required to analyze the organization of regenerative cells in the adult lung and identify the factors and molecular pathways which regulate their capacity to generate descendent lineages. In this review we describe recent progress in identifying and characterizing endogenous epithelial, mesenchymal and endothelial stem/progenitor cells in the adult lung using multiparameter cell separative strategies and functional in vitro clonogenic assays.
尽管人们对细胞疗法在肺再生医学中的潜力越来越感兴趣，但由于缺乏关于可用于修复肺部的合适靶点的知识，以及调节其再生潜力的细胞和分子因素，这些疗法的进展一直受到阻碍。虽然对正常和受干扰的动物模型中的肺发育和细胞谱系追踪研究进行系统分析为理解多种细胞类型、生物基质成分以及调节肺结构和功能的可溶性和不可溶性细胞因子和因素之间的复杂相互作用提供了一个框架，但还需要采用还原方法来分析成年肺部再生细胞的组织，并确定调节其产生后代谱系的因素和分子途径。在这篇综述中，我们描述了使用多参数细胞分离策略和体外克隆形成功能测定来鉴定和表征成年肺内源性上皮、间充质和内皮干细胞/祖细胞的最新进展。
