Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary.
J Sep Sci. 2012 Mar;35(5-6):617-24. doi: 10.1002/jssc.201100921. Epub 2012 Feb 14.
Direct separation of the enantiomers of amino acid amines, amino alcohols, and diamines was performed on recently developed chiral stationary phases containing isopropyl carbamate-cyclofructan 6 (IP-CF6), (R)-naphthylethylcarbamate cyclofructan 6 (RN-CF6), or dimethylphenylcarbamate cyclofructan 7 (DMP-CF7) as chiral selectors, using n-hexane/alcohol/TFA as mobile phase. The effects of the mobile phase composition, the nature and concentrations of the alcoholic and acidic modifiers, and the structures of the analytes on the retention and resolution were investigated. In some cases, separations were carried out at constant mobile phase composition in the temperature range 5-40 °C. Thermodynamic parameters and T(iso) values were calculated from plots of lnk versus 1/T. It was found that the enantioseparations were enthalpy driven. The sequences of elution of the stereoisomers were determined but no general rule could be established.
直接分离的对映异构体的氨基酸胺，氨基酸醇和二胺进行了最近开发的手性固定相含有异丙基氨基甲酸酯-cyclofructan 6 (IP-CF6)，（R）-萘乙基氨基甲酸酯 cyclofructan 6 (RN-CF6)，或二甲基氨基甲酸酯 cyclofructan 7 (DMP-CF7)作为手性选择器，使用正己烷/醇/TFA 作为流动相。研究了流动相组成、醇和酸改性剂的性质和浓度以及分析物的结构对保留和分离度的影响。在某些情况下，在 5-40°C 的温度范围内，在恒定的流动相组成下进行分离。从 lnk 对 1/T 的图中计算出热力学参数和 T(iso)值。结果发现，对映体分离是由焓驱动的。确定了立体异构体的洗脱顺序，但没有建立一般规则。
