应用反转DEPT极化转移脉冲序列通过1H NMR光谱研究灌注器官中13C标记底物的代谢。
Application of reverse-DEPT polarization transfer pulse sequence to study the metabolism of carbon-13-labeled substrates in perfused organs by 1H NMR spectroscopy.
作者信息
Desmoulin F, Confort-Gouny S, Masson S, Bernard M, Doddrell D M, Cozzone P J
机构信息
Centre de Résonance Magnétique Biologique et Médicale, URA CNRS-1186, Faculté de Médecine de la Timone, Marseille, France.
出版信息
Magn Reson Med. 1990 Sep;15(3):456-61. doi: 10.1002/mrm.1910150311.
PMID:2233224
Abstract
Metabolism of 13C-enriched metabolites can be advantageously studied by reverse-polarization transfer methods. In this work an improved reverse-DEPT sequence has been applied for the first time on perfused organs in a 20-mm probe. The metabolic fate of 99% enriched [2-13C]acetate perfused in excised rat liver and heart has been documented.
摘要
通过反向极化转移方法可以有效地研究富含¹³C的代谢物的代谢情况。在这项工作中，一种改进的反向DEPT序列首次应用于20毫米探头中的灌注器官。已记录了灌注到切除的大鼠肝脏和心脏中的99%富集的[2-¹³C]乙酸盐的代谢命运。
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