Shulepko M A, Liukmanova E N, Kasheverov I E, Dolgikh D A, Tsetlin V I, Kirpichnikov M P
Bioorg Khim. 2011 Sep-Oct;37(5):609-15. doi: 10.1134/s1068162011050165.
Lynx1 expresses in the central nervous system and plays important role in a regulation of nicotinic acetylcholine receptors. Successful milligram-quantitive expression of ws-Lynx1 was achieved only in the case of its production in the form of cytoplasm inclusion bodies. Different conditions of ws-Lynx1 refolding for yield optimization were performed. The obtained recombinant protein was characterized by means of mass spectrometry and CD spectroscopy. The binding experiments on the nAChRs from Torpedo californica membranes revealed that ws-Lynxl is biologically active and blocks muscle nAChR with IC50-20-30 microM.
Lynx1在中枢神经系统中表达，并在烟碱型乙酰胆碱受体的调节中发挥重要作用。仅在以细胞质包涵体形式产生ws-Lynx1的情况下，才成功实现了毫克级定量表达。对ws-Lynx1进行了不同的重折叠条件优化以提高产量。通过质谱和圆二色光谱对获得的重组蛋白进行了表征。对来自加州电鳐膜的烟碱型乙酰胆碱受体进行的结合实验表明，ws-Lynx1具有生物活性，可阻断肌肉烟碱型乙酰胆碱受体，其半数抑制浓度（IC50）为20 - 30微摩尔。
