Geng Xiaokun, Ren Changhong, Wang Tony, Fu Paul, Luo Yuming, Liu Xiangrong, Yan Feng, Ling Feng, Jia Jianping, Du Huishan, Ji Xunming, Ding Yuchuan
Xuanwu Hospital, Capital Medical University, Beijing, China.
Neurol Res. 2012 Mar;34(2):143-8. doi: 10.1179/1743132811Y.0000000073. Epub 2012 Jan 13.
While recent studies suggest that remote ischemic postconditioning (RIP) therapy may be of benefit to patients with acute ischemic stroke, RIP's effects on intracerebral hemorrhage (ICH) still remains unclear. In the present study, the use of RIP in a rat model ICH was investigated to elucidate any potential beneficial or detrimental effects as determined by motor testing, blood brain barrier integrity, and brain water content, as well as aquaporin-4 (AQP-4) and matrix metalloproteinase-9 (MMP-9) expression.
ICH was induced in Sprague-Dawley rats and they were randomized into either a control (n = 24) or RIP treatment (n = 24) group. RIP was performed by repetitive, brief occlusion and release of the bilateral femoral arteries. Functional outcome in each group was assessed by neurologic deficits on vibrissae-elicited forelimb placing test and a 12-point outcome scale. At 72 hours, brain blood volume, water content, blood-brain barrier (BBB) permeability, and protein expression of AQP-4 and MMP-9 were determined.
This collagenase model yielded well-defined striatal hematomas. Vibrissae-elicited forelimb placement was significantly (P<0·01) affected by ICH. However, there was no significant difference between the RIP and control groups at either 24 or 72 hours. A 12-point neurological deficit score also failed to differentiate between the RIP and control. There were no significant differences between the two groups in cerebral blood volumes, brain water content, Evans blue extravasations, and expressions of AQP-4 and MMP-9.
Although RIP did not show a beneficial effect in our ICH model, treatment with RIP did not exacerbate ICH.
虽然近期研究表明远程缺血后适应（RIP）疗法可能对急性缺血性脑卒中患者有益，但RIP对脑出血（ICH）的影响仍不清楚。在本研究中，研究了RIP在大鼠ICH模型中的应用，以通过运动测试、血脑屏障完整性、脑含水量以及水通道蛋白4（AQP-4）和基质金属蛋白酶9（MMP-9）表达来阐明任何潜在的有益或有害影响。
在Sprague-Dawley大鼠中诱导ICH，并将它们随机分为对照组（n = 24）或RIP治疗组（n = 24）。通过重复短暂阻断和松开双侧股动脉来进行RIP。通过触须诱发的前肢放置试验中的神经功能缺损和12分结果量表评估每组的功能结局。在72小时时，测定脑血容量、含水量、血脑屏障（BBB）通透性以及AQP-4和MMP-9的蛋白表达。
该胶原酶模型产生了明确的纹状体血肿。触须诱发的前肢放置受到ICH的显著影响（P<0·01）。然而，在24小时或72小时时，RIP组和对照组之间没有显著差异。12分神经功能缺损评分也未能区分RIP组和对照组。两组在脑血容量、脑含水量、伊文思蓝外渗以及AQP-4和MMP-9的表达方面没有显著差异。
虽然RIP在我们的ICH模型中未显示出有益效果，但RIP治疗并未加重ICH。
