[459例汉族肺结核患者中结核病易感性相关基因多态性与结核病临床特征的相关性]

[The correlation between polymorphisms of genes with susceptibility to tuberculosis and the clinical characteristics of tuberculosis in 459 Han patients].

作者信息

Liu Zhi-bin, Zheng Rui-juan, Xiao He-ping, Sha Wei, Zhang Qing, Wu Fu-rong, Sun Hua, Zhang Zhong-shun, Cui Hai-yan, Liu Yi-dian, Tang Shen-jie

机构信息

Shanghai Key Laboratory of Tuberculosis, Tuberculosis Diagnosis Center, Shanghai Pulmonary Hospital Affiliated to Tongji University, Shanghai 200433, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2011 Dec;34(12):923-8.

PMID:22333506

Abstract

OBJECTIVE

To study the correlation between polymorphisms of genes with susceptibility to tuberculosis and the clinical characteristics of tuberculosis in Han population.

METHODS

Four hundred and fifty-nine tuberculosis inpatients of Han population in Shanghai Pulmonary Hospital from Jan 2007 to Dec 2008 were recruited. The clinical characteristics of tuberculosis (gender, fever, extent of lesions, cavity formation, hemoptysis, initial treatment and retreatment) were observed. The polymorphisms of VDR gene (variants in FokI and TaqI), NRAMP1 gene (variants in INT4, D543N and 3 UTR), MBL gene (variants in HL, YX and QP) and IFNG gene (variants in 874AT) were genotyped by a variety of SNP genotyping techniques. The correlation between polymorphisms of genes with susceptibility to tuberculosis and the clinical characteristics of the disease was analyzed by ANOVAs.

RESULTS

The frequency of CC, CT and TT variants of FokI in VDR gene in cases with fever were 54.7% (29/53), 13.2% (7/53) and 32.1% (17/53), respectively, compared to 40.6% (52/128), 30.5% (39/128) and 28.9% (37/128) in cases without fever, the difference being significant (χ² = 6.183, P < 0.05). In patients with CT variants, 15.2% (7/46) had fever, while in patients with non-CT variants, 34.1% (46/135) had fever (χ² = 5.891, P < 0.05), suggesting that patients with CT variants were less likely to have fever. The frequencies of TT + TC and CC variants of QP in the MBL gene in initial treatment cases were 28.3% (60/212) and 71.7% (152/212), respectively, compared to 19.1% (41/215) and 80.9% (174/215) in retreatment cases, the difference being significant (χ² = 5.038, P < 0.05). No significant correlation was observed between the other variants and the clinical characteristics of tuberculosis (χ² = 0.001 - 2.732, P > 0.05).

CONCLUSIONS

The polymorphisms of FokI in VDR gene was associated with fever among the clinical characteristics of tuberculosis, and patients with CT variants might be protected from fever. The polymorphisms of QP in MBL gene might be associated with recurrence of tuberculosis.

摘要

目的

研究汉族人群中与结核病易感性相关基因的多态性与结核病临床特征之间的相关性。

方法

选取2007年1月至2008年12月在上海肺科医院住院的459例汉族结核病患者。观察结核病的临床特征（性别、发热、病变范围、空洞形成、咯血、初治和复治）。采用多种单核苷酸多态性基因分型技术对维生素D受体（VDR）基因（FokI和TaqI位点的变异）、天然抵抗相关巨噬蛋白1（NRAMP1）基因（INT4、D543N和3′非翻译区的变异）、甘露聚糖结合凝集素（MBL）基因（HL、YX和QP位点的变异）和干扰素-γ（IFNG）基因（874A/T位点的变异）进行基因分型。通过方差分析分析与结核病易感性相关基因的多态性与疾病临床特征之间的相关性。

结果

发热患者中VDR基因FokI位点CC、CT和TT变异的频率分别为54.7%（29/53）、13.2%（7/53）和32.1%（17/53），无发热患者中分别为40.6%（52/128）、30.5%（39/128）和28.9%（37/128），差异有统计学意义（χ² = 6.183，P < 0.05）。CT变异患者中15.2%（7/46）有发热，非CT变异患者中34.1%（46/135）有发热（χ² = 5.891，P < 0.05），提示CT变异患者发热可能性较小。初治患者中MBL基因QP位点TT + TC和CC变异的频率分别为28.3%（60/212）和71.7%（152/212），复治患者中分别为19.1%（41/215）和80.9%（174/215），差异有统计学意义（χ² = 5.038，P < 0.05）。其他变异与结核病临床特征之间未观察到显著相关性（χ² = 0.001 - 2.732，P > 0.05）。