Edamura Kazuya, Kuriyama Koji, Kato Kenichi, Nakano Rei, Teshima Kenji, Asano Kazushi, Sato Tsuneo, Tanaka Shigeo
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
J Vet Med Sci. 2012 Jul;74(7):923-7. doi: 10.1292/jvms.11-0388. Epub 2012 Feb 15.
We examined the proliferation capacity and neuronal differentiation potency of canine bone marrow stromal cells (BMSCs). In addition, the microstructures of neuron-like cells after neuronal differentiation were observed under a scanning electron microscope. Canine BMSCs grew to confluency at 10.0 ± 2.5 days, and 3.8 ± 2.1 × 10(6) BMSCs were collected in one passage. Approximately 65% of canine BMSCs changed to neuron-like morphology after neuronal differentiation, and nearly all neuron-like cells stained positive against neuron-specific enolase. In addition, microstructures such as the cellular organelles, filaments and growth cones of these cells bore a close resemblance to those of the original mature neurons. These results suggested that canine BMSCs might be capable of differentiating into neurons.
我们检测了犬骨髓基质细胞（BMSCs）的增殖能力和神经元分化潜能。此外，在扫描电子显微镜下观察了神经元分化后类神经元细胞的微观结构。犬BMSCs在10.0±2.5天达到汇合，每代收集3.8±2.1×10(6)个BMSCs。神经元分化后，约65%的犬BMSCs转变为类神经元形态，几乎所有类神经元细胞对神经元特异性烯醇化酶染色呈阳性。此外，这些细胞的细胞器、细丝和生长锥等微观结构与原始成熟神经元的微观结构极为相似。这些结果表明犬BMSCs可能能够分化为神经元。
