Edamura Kazuya, Kurosawa Takashi, Nakano Rei, Teshima Kenji, Asano Kazushi, Tanaka Shigeo
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
J Vet Med Sci. 2012 Jun;74(6):817-20. doi: 10.1292/jvms.11-0367. Epub 2012 Jan 27.
We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5, 10 and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.
我们研究了添加自体血清（AS）的培养基对犬骨髓间充质干细胞（BMSC）增殖及向神经元分化的影响。犬BMSC分别用仅含α-MEM、含10%胎牛血清（FBS）的α-MEM以及添加5%、10%和20% AS的α-MEM进行培养。在所有添加AS的组中均观察到犬BMSC的生长。添加AS组中犬BMSC的增殖能力与FBS组相似。在神经元分化后，转变为类神经元形态的细胞百分比及神经元特异性烯醇化酶阳性率在FBS组和添加AS组之间未观察到显著差异。使用添加AS的培养基培养的犬BMSC能够增殖并表现出神经元分化潜能。
