Dongguk University Research Institute of Biotechnology Medical Science Research Center, Gyeonggi-do, Korea.
Acta Derm Venereol. 2012 Nov;92(6):675-80. doi: 10.2340/00015555-1319.
Treatment of hyperpigmentation remains a challenge. Because of the positive effects of low-energy Nd:YAG lasers on the treatment of melasma, it is suggested that laser-like light-emitting diodes (LEDs) can potentially ameliorate hyperpigmentation. We evaluated the effect of seven different LED wavelengths on melanogenesis. LED irradiation at 830 nm (dose-dependent, from 1 to 20 J/cm2) and 850 nm (1 J/cm2) significantly reduced melanin production and tyrosinase expression, not only in a normal human melanocyte monoculture both with and without forskolin stimulation but also in a three-dimensional multiple cell type culture. It reduced melanin content via inactivation of the apoptosis signal-regulating kinase and extracellular signal-regulated kinase 1/2 pathways. The level of phosphorylated cyclic AMP response element-binding protein was also decreased by LED irradiation. Moreover, LED irradiation reduced melanogenesis through decreased expression of tyrosinase family genes (tyrosinase-related protein-1 and 2, and microphthalmia-associated transcription factor). These results indicate that LEDs could potentially be used to treat melanin-overproducing skin conditions.
治疗色素沉着仍然是一个挑战。由于低能量钕：YAG 激光对黄褐斑治疗的积极作用，有人提出激光样发光二极管（LED）有可能改善色素沉着。我们评估了七种不同 LED 波长对黑色素生成的影响。830nm（剂量依赖性，从 1 到 20J/cm2）和 850nm（1J/cm2）的 LED 辐射显著降低了黑色素生成和酪氨酸酶表达，不仅在正常的人类黑素细胞单层培养物中（无论是否有 forskolin 刺激），而且在三维多细胞培养物中也是如此。它通过失活凋亡信号调节激酶和细胞外信号调节激酶 1/2 通路来减少黑色素含量。LED 辐射还降低了磷酸化环 AMP 反应元件结合蛋白的水平。此外，LED 辐射通过降低酪氨酸酶家族基因（酪氨酸酶相关蛋白-1 和 2，以及小眼畸形相关转录因子）的表达来减少黑色素生成。这些结果表明，LED 有可能用于治疗黑色素过度生成的皮肤状况。
